Gene Expression Data
Assay Details
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Assay
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Reference:
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J:46971
Loomis CA, et al., Analysis of the genetic pathway leading to formation of ectopic apical ectodermal ridges in mouse Engrailed-1 mutant limbs. Development. 1998 Mar;125(6):1137-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1339623
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Gene symbol:
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Lmx1b
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Gene name:
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LIM homeobox transcription factor 1 beta
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Probe:
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Lmx1b cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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All the specimens were discussed in the paper as in limb bud stages. Some assay results are only annotated to forelimb buds, but they could also be annotated to the same limb bud stage hindlimbs of a later embryonic date.
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Results
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Specimen
1J:
embryonic day 9.5
(more  )
Note: Limb-bud stage (stage 1).
Specimen
1J:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 1).
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Specimen
1K:
embryonic day 9.5
(more )
Note: Limb-bud stage (stage 1).
Specimen
1K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Limb-bud stage (stage 1).
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Specimen
1L:
embryonic day 10.5
(more )
Note: Limb-bud stage (stage 3).
Specimen
1L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Limb-bud stage (stage 3).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb mesenchyme |
Present |
Regionally restricted |
1L
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Expression is restricted to the dorsal mesenchyme.
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Specimen
2I:
embryonic day 10.5; En1tm2Alj/En1 +
(more )
Note: Limb-bud stage (stage 3/4). Double labeled: purple - Lmx1b; pink - En1 reporter (assay MGI:5318340).
Specimen
2I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4). Double labeled: purple - Lmx1b; pink - En1 reporter (assay MGI:5318340).
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Specimen
2J:
embryonic day 10.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 3/4). Double labeled: purple - Lmx1b; pink - En1 reporter (assay MGI:5318340).
Specimen
2J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 3/4). Double labeled: purple - Lmx1b; pink - En1 reporter (assay MGI:5318340).
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Specimen
3E:
embryonic day 11.5
(more )
Note: Limb-bud stage (stage 5/6).
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 5/6).
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Specimen
3F:
embryonic day 11.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 5/6).
Specimen
3F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 5/6).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb mesenchyme |
Present |
Regionally restricted |
3F
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In mutants there is ectopic reexpression in the ventral mesenchyme.
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Specimen
3I:
embryonic day 11.5-12.5; Wnt7atm1Amc/ Wnt7atm1Amc
(more )
Note: Limb-bud stage (stage 7/8). Double labeled: purple - Lmx1b; pink - En1 reporter (assay MGI:5318340).
Specimen
3I:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5-12.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 7/8). Double labeled: purple - Lmx1b; pink - En1 reporter (assay MGI:5318340).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
3I
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In the stage 5/6 hindlimb expression is relatively normal in the cells nearly abutting the apical ectodermal ridge.
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| TS20: forelimb |
Present |
Regionally restricted |
3I
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In the 7/8 forelimb, there is no expression in the distodorsal mesenchyme adjacent to the ridge.
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Specimen
4I:
embryonic day 13.5-14.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 10). Double labeled: purple - Lmx1b; pink - En1 reporter (assay MGI:5318340).
Specimen
4I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 13.5-14.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 10). Double labeled: purple - Lmx1b; pink - En1 reporter (assay MGI:5318340).
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Specimen
4J:
embryonic day 13.5-14.5; En1tm2Alj/ En1tm2Alj
(more )
Note: Limb-bud stage (stage 10).
Specimen
4J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
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Age:
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embryonic day 13.5-14.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 10).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: limb |
Present |
Regionally restricted |
4J
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Expression has cleared from the posterior ventral area. Clearing is irregular compared to wild-type embryos.
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1tm2Alj
Wnt7atm1Amc/Wnt7atm1Amc
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Limb-bud stage (stage 5/6).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb mesenchyme |
Present |
Regionally restricted |
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No expression in the distodorsal mesenchyme adjacent to the ridge. No ectopic ventral expression.
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Analysis Tools