Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:34586
Wehrle-Haller B, et al., Ectopic c-kit expression affects the fate of melanocyte precursors in Patch mutant embryos. Dev Biol. 1996 Aug 1;177(2):463-74
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:1339494
|
|
Gene symbol:
|
Kitl
|
|
Gene name:
|
kit ligand
|
|
Antibody:
|
ANTI-SLF
|
|
Detection system:
|
Secondary antibody coupled to alkaline phosphatase
|
|
|
Results
|
Specimen
5A:
embryonic day 10.5
(more  )
Note: 35-36 somites. Lateral view.
Specimen
5A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
35-36 somites. Lateral view.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Present |
Regionally restricted |
5A
|
In the trunk, expression was in the dorsal aspect of the epithelial dermatome.
|
Specimen
5F:
embryonic day 10.5; Ph/ Ph
(more )
Note: 35-36 somites. Lateral view.
Specimen
5F:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6
|
|
Mutant Allele(s):
|
Ph/Ph
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
35-36 somites. Lateral view.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk dermomyotome |
Present |
Regionally restricted |
5F
|
In the trunk, expression was in the dorsal aspect of the epithelial dermatome.
|
Specimen
5B:
embryonic day 11.0
(more )
Note: 39-40 somites. Lateral view at the forelimb level.
Specimen
5B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
39-40 somites. Lateral view at the forelimb level.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk dermomyotome |
Present |
Regionally restricted |
5B
|
Expression is in the dorsal aspects at the hind limb. Anteriorly, expression in the epithelial dermatome decreases as it transforms to mesenchymal dermis.
|
Specimen
5G:
embryonic day 11.0; Ph/ Ph
(more )
Note: 39-40 somites. Lateral view at the forelimb level.
Specimen
5G:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6
|
|
Mutant Allele(s):
|
Ph/Ph
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
39-40 somites. Lateral view at the forelimb level.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk dermomyotome |
Present |
Regionally restricted |
5G
|
Expression is in the dorsal aspects at the hind limb. Anteriorly, expression in the epithelial dermatome decreases as it transforms to mesenchymal dermis.
|
Specimen
5C,D,E:
embryonic day 11.0
(more )
Note: 39-40 somites. The plane of the section is shown in (B). (D) corresponds to the epithelial dorsal dermatome, (E) corresponds to the mesenchymal mediolateral aspect of the dermis.
Specimen
5C,D,E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
39-40 somites. The plane of the section is shown in (B). (D) corresponds to the epithelial dorsal dermatome, (E) corresponds to the mesenchymal mediolateral aspect of the dermis.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: trunk mesenchyme |
Weak |
Regionally restricted |
5C
,
5E
,
5D
|
Expression is in newly transformed dermal mesenchyme.
|
Specimen
5H,I,J:
embryonic day 11.0; Ph/ Ph
(more )
Note: 39-40 somites. The plane of the section is shown in (G). (I) corresponds to the epithelial dorsal dermatome, (J) corresponds to the mesenchymal mediolateral aspect of the dermis.
Specimen
5H,I,J:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6
|
|
Mutant Allele(s):
|
Ph/Ph
|
|
Age:
|
embryonic day 11.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
39-40 somites. The plane of the section is shown in (G). (I) corresponds to the epithelial dorsal dermatome, (J) corresponds to the mesenchymal mediolateral aspect of the dermis.
|
|
Analysis Tools