Gene Expression Data
Assay Details
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Assay
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Reference:
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J:27786
Chan DC, et al., Polydactylous limbs in Strong's Luxoid mice result from ectopic polarizing activity. Development. 1995 Jul;121(7):1971-8
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1336681
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 11.5
(more  )
Note: Also stained for Fgf4 (assay MGI:1336680).
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Also stained for Fgf4 (assay MGI:1336680).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
2A
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Expression is in the zone of polarizing activity.
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| TS19: hindlimb bud |
Present |
Regionally restricted |
2A
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Expression is in the zone of polarizing activity.
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Specimen
2B:
embryonic day 11.5; Alx4lst/Alx4 +
(more )
Note: Also stained for Fgf4 (assay MGI:1336680).
Specimen
2B:
(close )
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Genetic Background:
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involves: NHO/Brpb
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Mutant Allele(s):
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Alx4lst/Alx4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Also stained for Fgf4 (assay MGI:1336680).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
2B
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Expression in this mutant is the same as in wild-type embryos.
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| TS19: hindlimb bud |
Present |
Regionally restricted |
2B
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This mutant shows ectopic expression in a small patch of anterior mesoderm that underlies the ectopic Fgf4 expression. Expression is also seen in the zone of polarizing activity.
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Specimen
2C:
(close )
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Genetic Background:
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involves: NHO/Brpb
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Mutant Allele(s):
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Alx4lst/Alx4lst
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Also stained for Fgf4 (assay MGI:1336680).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
2C
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This mutant shows ectopic expression in the anterior regions underlying the ectopic expression of Fgf4. This ectopic expression is later than that of Fgf4. Normal expression is also seen in the zone of polarizing activity.
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| TS19: hindlimb bud |
Present |
Regionally restricted |
2C
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This mutant shows ectopic expression in the anterior regions underlying the ectopic expression of Fgf4. This ectopic expression is later than that of Fgf4. Normal expression is also seen in the zone of polarizing activity.
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| TS19: footplate mesenchyme |
Present |
Regionally restricted |
2C
,
2E
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Mutant embryos show ectopic expression in the mesodermal cells that underlie the cells ectopically expressing Fgf4.
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| TS19: handplate mesenchyme |
Present |
Regionally restricted |
2C
,
2E
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Mutant embryos show ectopic expression in the mesodermal cells that underlie the cells ectopically expressing Fgf4.
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Specimen
2G:
(close )
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Genetic Background:
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involves: NHO/Brpb
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Mutant Allele(s):
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Alx4lst/Alx4lst
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: footplate mesenchyme |
Present |
Regionally restricted |
2G
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There is ectopic expression in the anterior mesoderm in mutant embryos.
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| TS19: handplate mesenchyme |
Present |
Regionally restricted |
2G
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There is ectopic expression in the anterior mesoderm in mutant embryos.
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Analysis Tools