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Gene Expression Data
Assay Details
Assay
Reference: J:27786 Chan DC, et al., Polydactylous limbs in Strong's Luxoid mice result from ectopic polarizing activity. Development. 1995 Jul;121(7):1971-8
Assay type: RNA in situ
MGI Accession ID: MGI:1336680
Gene symbol: Fgf4
Gene name: fibroblast growth factor 4
Probe: Fgf-4 cDNA1
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 2A: embryonic day 11.5 (more )
Note: Also stained for Shh (assay MGI:1336681).
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Present Regionally restricted 2A Expression is in the central and posterior portion.
TS19: handplate apical ectodermal ridge Present Regionally restricted 2A Expression is in the central and posterior portion.
Specimen 2B: embryonic day 11.5; Alx4lst/Alx4+ (more )
Note: Also stained for Shh (assay MGI:1336681).
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present Regionally restricted 2B Expression in this mutant is the same as in wild-type embryos. Expression is in the central and posterior portion of the apical ectodermal ridge.
TS19: footplate apical ectodermal ridge Present Regionally restricted 2B Mutant embryos have ectopic expression in the anterior apical ectodermal ridge in additoin to the normal expression in the central and posterior regions.
Specimen 2C: embryonic day 11.5; Alx4lst/Alx4lst (more )
Note: Also stained for Shh (assay MGI:1336681).
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Present Regionally restricted 2C Mutant animals show ectopic, anterior expression in all limbs. Expression in the posterior and central portions is normal.
TS19: handplate apical ectodermal ridge Present Regionally restricted 2C Mutant animals show ectopic, anterior expression in all limbs. Expression in the posterior and central portions is normal.
Specimen 2D: embryonic day 10.5; Alx4lst/Alx4lst (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present Regionally restricted 2D This mutant shows ectopic expression in the anterior apical ectodermal ridge as well as normal expression in the central and posterior regions.
Specimen 2E: embryonic day 11.5; Alx4lst/Alx4lst (more )
Note: Also stained for Shh (assay MGI:1336681).
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Present Regionally restricted 2E Mutant animals show ectopic, anterior expression in all limbs. Expression in the posterior and central portions is normal.
TS19: handplate apical ectodermal ridge Present Regionally restricted 2E Mutant animals show ectopic, anterior expression in all limbs. Expression in the posterior and central portions is normal.
Specimen 2F: embryonic day 11.5; Alx4lst/Alx4lst (more )
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Present Regionally restricted 2F This mutant shows ectopic expression in the anterior apical ectodermal ridge.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/28/2024
MGI 6.13 		The Jackson Laboratory