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Gene Expression Data
Assay Details
Assay
Reference: J:30345 Haramis AG, et al., The limb deformity mutation disrupts the SHH/FGF-4 feedback loop and regulation of 5' HoxD genes during limb pattern formation. Development. 1995 Dec;121(12):4237-45
Assay type: RNA in situ
MGI Accession ID: MGI:1336020
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh cDNA3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4A: embryonic day 10.25 (more )
Note: 32 somites.
Structure Level Pattern Image Note
TS16: neural tube floor plate Present 4A
TS16: notochord Present 4A
TS16: forelimb bud mesenchyme Present Regionally restricted 4A Expression is in the proximal posterior mesenchyme.
Specimen 4B: embryonic day 10.25; Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Note: 33 somites.
Structure Level Pattern Image Note
TS16: neural tube floor plate Present 4B
TS16: notochord Present 4B
TS16: forelimb bud Present Regionally restricted 4B Expression is in the proximal posterior mesenchyme.
Specimen 4C: embryonic day 10.5; Fmn1ld-Is(17;In2)1Gso/Fmn1+ (more )
Note: 35 somites.
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 4C
TS17: notochord Present 4C
TS17: forelimb bud mesenchyme Strong Regionally restricted 4C The proximal-posterior domain of expression expands distally.
Specimen 4D: embryonic day 10.5; Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Note: 35 somites.
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 4D
TS17: notochord Present 4D
TS17: forelimb bud mesenchyme Present Regionally restricted 4D The proximal posterior domain of expression begins to extend distally. Expression in this mutant is the same as in wild-type embryos.
Specimen 4E: embryonic day 10.75; Fmn1ld-Is(17;In2)1Gso/Fmn1+ (more )
Note: 38 somites.
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 4E
TS17: notochord Present 4E
TS17: forelimb bud mesenchyme Strong Regionally restricted 4E Expression increases in the distal part of the posterior limb bud mesenchyme.
Specimen 4F: embryonic day 10.75; Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso (more )
Note: 38 somites.
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 4F
TS17: notochord Present 4F
TS17: forelimb bud mesenchyme Absent 4F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/28/2026
MGI 6.24 		The Jackson Laboratory