Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:30345
Haramis AG, et al., The limb deformity mutation disrupts the SHH/FGF-4 feedback loop and regulation of 5' HoxD genes during limb pattern formation. Development. 1995 Dec;121(12):4237-45
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1336005
|
|
Gene symbol:
|
Hoxd11
|
|
Gene name:
|
homeobox D11
|
|
Probe:
|
Hoxd11 probe7
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
2A:
(close  )
|
Genetic Background:
|
involves: 101/Rl * C3H/Rl
|
|
Mutant Allele(s):
|
Fmn1ld-Is(17;In2)1Gso/Fmn1+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
35 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
2A
|
Anterior expansion of the initial proximal-posterior expression has already begun at this stage.
|
Specimen
2D:
(close )
|
Genetic Background:
|
involves: 101/Rl * C3H/Rl
|
|
Mutant Allele(s):
|
Fmn1ld-Is(17;In2)1Gso/Fmn1ld-Is(17;In2)1Gso
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
35 somites.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
2D
|
Expression is in the proximal-posterior region of the limb. Anterior expansion has not begun.
|
|
Analysis Tools
)
