Gene Expression Data
Assay Details
Assay
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Reference:
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J:24364
Burgess R, et al., Paraxis: a basic helix-loop-helix protein expressed in paraxial mesoderm and developing somites. Dev Biol. 1995 Apr;168(2):296-306
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1334726
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Gene symbol:
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Tcf15
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Gene name:
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transcription factor 15
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Probe:
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Paraxis probe-1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2.A & B:
embryonic day 7.5
(more )
Specimen
2.A & B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo mesoderm |
Weak |
Regionally restricted |
2A/B
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Expression is restricted to the primative mesoderm in the posterior region of the embryo.
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Specimen
3.A & B:
embryonic day 8.5
(more )
Specimen
3.A & B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS13: head paraxial mesenchyme |
Present |
Regionally restricted |
3A/B
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Expression in the paraxial mesoderm and newly formed somites progressed caudally between 8.0 and 12.5 dpc.
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Specimen
3.C & D:
embryonic day 9.5
(more )
Specimen
3.C & D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS15: trunk somite |
Present |
Regionally restricted |
3C/D
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Expression is found in the dermatome, myotome, and sclerotome of somites that have recently compartmentalized.
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Specimen
3.E & F:
embryonic day 9.5
(more )
Specimen
3.E & F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3.G & H:
embryonic day 9.5
(more )
Specimen
3.G & H:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS15: trunk somite |
Present |
Regionally restricted |
3G/H
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Expression is present in newly-formed caudal somites (at bottom) and is down-regulated in myotome of mature rostral somites(at top).
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Specimen
3.I:
embryonic day 10.5
(more )
Specimen
3.I:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk somite |
Present |
Regionally restricted |
3I
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Strong expression is detected in the lateral sclerotomes; weak expression is seen in myotomes.
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TS17: forelimb bud |
Present |
Regionally restricted |
3I
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Expression is localized to the dorsal region of the forelimb.
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Specimen
5.B:
embryonic day 10.5
(more )
Specimen
5.B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS17: trunk paraxial mesenchyme |
Present |
Regionally restricted |
5B
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Transcripts are localized to somites.
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TS17: limb |
Present |
Regionally restricted |
5B
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Expression is localized to cells in the proximal region of the limb bud.
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