Gene Expression Data
Assay Details
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Assay
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Reference:
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J:19212
Wurst W, et al., Multiple developmental defects in Engrailed-1 mutant mice: an early mid-hindbrain deletion and patterning defects in forelimbs and sternum. Development. 1994 Jul;120(7):2065-75
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1334708
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Gene symbol:
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En2
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Gene name:
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engrailed 2
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Antibody:
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alpha Enhb-1
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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The antibody used in this assay cross-reacts with both EN1 and EN2 proteins.
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Results
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Specimen
4H:
embryonic day 9.5
(more  )
Specimen
4H:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
4H
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Expression is in a wedge at the mid-hindbrain junction. Other signal is due to the antibody cross-reacting with EN1 protein.
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| TS15: future midbrain |
Present |
Regionally restricted |
4H
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Expression is in a wedge at the mid-hindbrain junction. Other signal is due to the antibody cross-reacting with EN1 protein.
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Specimen
4I:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J)
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Mutant Allele(s):
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En1tm1Alj/En1tm1Alj
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
4I
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In mutants, expression is in dorsal and ventral patches at the mid-hindbrain junction.
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| TS15: future midbrain |
Present |
Regionally restricted |
4I
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In mutants, expression is in dorsal and ventral patches at the mid-hindbrain junction.
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Analysis Tools
