Gene Expression Data
Assay Details
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Assay
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Reference:
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J:17224
Bober E, et al., Pax-3 is required for the development of limb muscles: a possible role for the migration of dermomyotomal muscle progenitor cells. Development. 1994 Mar;120(3):603-12
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1333919
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Probe:
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Myf5 probe2
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Probe preparation:
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labelled with S35 RNA
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Results
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Specimen
4B/F/J:
embryonic day 10.5
(more  )
Specimen
4B/F/J:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: trunk myotome |
Present |
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4F/J
,
4B
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Myf-5 transcripts were confined to the myotomal layer of somites.
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| TS17: forelimb bud |
Present |
Regionally restricted |
4B
,
4F/J
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Similar expression domains for Pax-3 and Myf-5 were observed in two locations of forelimbs at the ventral and dorsal sides, which later give rise to the flexor and extensor muscles.
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Specimen
4D/H/L:
embryonic day 10.5
(more )
Specimen
4D/H/L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
E9.5 control:
embryonic day 9.5
(more )
Specimen
E9.5 control:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
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Myf-5 was expressed at similar levels in axial and body wall muscles of control and mutant animals.
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| TS15: limb |
Strong |
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Specimen
E9.5 homozygous Sp1H:
(close )
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Genetic Background:
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involves: 101/H * C3H * C3H/HeH
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Mutant Allele(s):
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Pax3Sp-1H/Pax3Sp-1H
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
Regionally restricted |
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Myf-5 was expressed at similar levels in axial and body wall muscles of control and mutant animals.
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| TS15: limb |
Absent |
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Specimen
E10.5 control:
embryonic day 10.5
(more )
Specimen
E10.5 control:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
Regionally restricted |
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Myf-5 was expressed at similar levels in axial and body wall muscles of control and mutant animals.
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| TS17: limb |
Strong |
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Specimen
E10.5 homozygous Sp1H:
(close )
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Genetic Background:
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involves: 101/H * C3H * C3H/HeH
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Mutant Allele(s):
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Pax3Sp-1H/Pax3Sp-1H
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
Regionally restricted |
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Myf-5 was expressed at similar levels in axial and body wall muscles of control and mutant animals.
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| TS17: limb |
Absent |
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Analysis Tools