Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:43311
Peichel CL, et al., The mouse Ulnaless mutation deregulates posterior HoxD gene expression and alters appendicular patterning. Development. 1997 Sep;124(18):3481-92
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1333596
|
|
Gene symbol:
|
Hoxd12
|
|
Gene name:
|
homeobox D12
|
|
Probe:
|
Hox-4.7 probe2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
See the Materials and Methods for details of the possible strain backgrounds.
|
|
|
Results
|
Specimen
2B +/+ upper:
embryonic day 11.0
(more  )
Specimen
2B +/+ upper:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
Regionally restricted |
2B +/+ upper
|
Expression is in the autopod (digits), with weaker expression in a more proximal and posterior region.
|
Specimen
2B +/+ lower:
embryonic day 11.0
(more )
Specimen
2B +/+ lower:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
2B +/+ lower
|
Expression is in the autopod (digits), with weaker expression in a more proximal and posterior region.
|
Specimen
2B Ul/+ upper:
embryonic day 11.0; LnpkUl/Lnpk +
(more )
Specimen
2B Ul/+ upper:
(close )
|
Genetic Background:
|
either: (involves: FVB/N) or (involves: MOLF/Ei)
|
|
Mutant Allele(s):
|
LnpkUl/Lnpk+
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
Regionally restricted |
2B Ul/+ upper
|
Expression is in the autopod (digits) and expression is expanded proximally and anteriorly.
|
Specimen
2B Ul/+ lower:
embryonic day 11.0; LnpkUl/Lnpk +
(more )
Specimen
2B Ul/+ lower:
(close )
|
Genetic Background:
|
either: (involves: FVB/N) or (involves: MOLF/Ei)
|
|
Mutant Allele(s):
|
LnpkUl/Lnpk+
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
2B Ul/+ lower
|
Expression is in the autopod (digits) and expression is expanded proximally and anteriorly.
|
Specimen
2B Ul/Ul upper:
embryonic day 11.0; LnpkUl/ LnpkUl
(more )
Specimen
2B Ul/Ul upper:
(close )
|
Genetic Background:
|
either: (involves: FVB/N) or (involves: MOLF/Ei)
|
|
Mutant Allele(s):
|
LnpkUl/LnpkUl
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb |
Present |
Regionally restricted |
2B Ul/Ul upper
|
There is a reduction in expression distally and an expansion of expression proximaly and anteriorly.
|
Specimen
2B Ul/Ul lower:
embryonic day 11.0; LnpkUl/ LnpkUl
(more )
Specimen
2B Ul/Ul lower:
(close )
|
Genetic Background:
|
either: (involves: FVB/N) or (involves: MOLF/Ei)
|
|
Mutant Allele(s):
|
LnpkUl/LnpkUl
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference. Theiler stage of this specimen assigned by curators from EMAGE.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
Regionally restricted |
2B Ul/Ul lower
|
There is a reduction in expression distally and an expansion of expression proximaly and anteriorly.
|
Specimen
5B +/+:
embryonic day 11.0
(more )
Specimen
5B +/+:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5B Ul/+:
embryonic day 11.0; LnpkUl/Lnpk +
(more )
Specimen
5B Ul/+:
(close )
|
Genetic Background:
|
either: (involves: FVB/N) or (involves: MOLF/Ei)
|
|
Mutant Allele(s):
|
LnpkUl/Lnpk+
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5B Ul/Ul:
embryonic day 11.0; LnpkUl/ LnpkUl
(more )
Specimen
5B Ul/Ul:
(close )
|
Genetic Background:
|
either: (involves: FVB/N) or (involves: MOLF/Ei)
|
|
Mutant Allele(s):
|
LnpkUl/LnpkUl
|
|
Age:
|
embryonic day 11.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Analysis Tools