Probe:	Gbx2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present	Regionally restricted	6C	There is a stripe of Gbx2 expression in posterior neuroectoderm cells that expresses neither Hoxb1 nor Otx2.
TS11: embryo	Present	Regionally restricted	6C	Expressed in all three germ layers in a domain similar to that of Hoxb1, but extending slightly further rostrally, abutting the posterior boundary of the Otx2 expression domain.

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Regionally restricted	6D	Expression is at the posterior end of the embryo.
TS13: branchial arch	Present		6D
TS13: future hindbrain	Present	Regionally restricted	6D	Epression is in a broad stripe at the most anterior end of the hindbrain.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		6E
TS15: future hindbrain	Present	Regionally restricted	6E	Epression is in a broad stripe at the most anterior end.
TS15: tail	Present	Regionally restricted	6E	Expression is at the tail bud.
TS15: branchial arch	Present		6E

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present		6F
TS17: limb	Present		6F
TS17: otocyst	Present		6F
TS17: tail	Present		6F
TS17: midbrain	Present	Regionally restricted	6F	Expression is in a sharp transverse ring immediately caudal to the midbrain.
TS17: hindbrain	Present	Regionally restricted	6F	Expression is in longitudinal stripes that run the length of the hindbrain and spinal cord.
TS17: spinal component of peripheral nervous system	Present	Regionally restricted	6F	Expression is in longitudinal stripes that run the length of the hindbrain and spinal cord.