Gene Expression Data
Assay Details
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Assay
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Reference:
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J:42110
Wassarman KM, et al., Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development. 1997 Aug;124(15):2923-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1331426
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Gene symbol:
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Otx2
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Gene name:
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orthodenticle homeobox 2
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Probe:
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Otx2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3C:
embryonic day 10.5
(more  )
Specimen
3C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 4th ventricle |
Present |
Regionally restricted |
3C
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Expression is throughout the midbrain and at the roof of the 4th ventricle (choroid plexus), but is not detected in the rest of the hindbrain.
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| TS17: hindbrain |
Present |
Regionally restricted |
3C
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Expression is throughout the midbrain and at the roof of the 4th ventricle (choroid plexus), but is not detected in the rest of the hindbrain.
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| TS17: midbrain |
Present |
Regionally restricted |
3C
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Expression is throughout the midbrain and at the roof of the 4th ventricle (choroid plexus), but is not detected in the rest of the hindbrain.
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Specimen
3D:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2J) or (involves: 129S1/Sv * 129X1/SvJ * FVB/N)
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 4th ventricle |
Present |
Regionally restricted |
3D
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The non-expressing region in the anterior hindbrain is severely reduced.
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| TS17: hindbrain |
Present |
Regionally restricted |
3D
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The non-expressing region in the anterior hindbrain is severely reduced.
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| TS17: midbrain |
Present |
Regionally restricted |
3D
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The non-expressing region in the anterior hindbrain is severely reduced.
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Specimen
4E:
embryonic day 10.5
(more )
Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 4th ventricle |
Present |
Regionally restricted |
4E
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Expression is present in the roof of the hindbrain, marking the prospective choroid plexus.
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| TS17: hindbrain |
Present |
Regionally restricted |
4E
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Expression is present in the roof of the hindbrain, marking the prospective choroid plexus.
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| TS17: midbrain |
Present |
Regionally restricted |
4E
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Expression is present in the roof of the hindbrain, marking the prospective choroid plexus.
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Specimen
4F:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2J) or (involves: 129S1/Sv * 129X1/SvJ * FVB/N)
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Mutant Allele(s):
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Gbx2tm1.1Mrt/Gbx2tm1.1Mrt
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 4th ventricle |
Present |
Regionally restricted |
4F
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Expression is present in the roof of the hindbrain, marking the prospective choroid plexus. In mutant embryos, the non-expressing region in the anterior hindbrain is severely reduced.
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| TS17: hindbrain |
Present |
Regionally restricted |
4F
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Expression is present in the roof of the hindbrain, marking the prospective choroid plexus. In mutant embryos, the non-expressing region in the anterior hindbrain is severely reduced.
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| TS17: midbrain |
Present |
Regionally restricted |
4F
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Expression is present in the roof of the hindbrain, marking the prospective choroid plexus. In mutant embryos, the non-expressing region in the anterior hindbrain is severely reduced.
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Specimen
6A:
embryonic day 7.75
(more )
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: neural ectoderm |
Present |
Regionally restricted |
6A
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The posterior boundary of expression marks the posterior end of the midbrain.
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