Gene Expression Data
Assay Details
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Assay
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Reference:
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J:42445
Dunn NR, et al., Haploinsufficient phenotypes in Bmp4 heterozygous null mice and modification by mutations in Gli3 and Alx4. Dev Biol. 1997 Aug 15;188(2):235-47
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1313182
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh clone
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6. A:
embryonic day 11.5
(more  )
Specimen
6. A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6. B:
embryonic day 11.5
(more )
Specimen
6. B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6. C:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Alx4tm1Rwi/Alx4+
Bmp4tm1Blh/Bmp4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future leg mesenchyme |
Weak |
Regionally restricted |
6C
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Expression is detected in the mesenchyme along the posterior margin of the limb bud.
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| TS19: footplate mesenchyme |
Weak |
Regionally restricted |
6C
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A small focus of expression is sometimes seen in the anterior mesenchyme
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Specimen
6. D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Alx4tm1Rwi/Alx4+
Bmp4tm1Blh/Bmp4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future leg mesenchyme |
Weak |
Regionally restricted |
6D
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Expression is detected in the mesenchyme along the posterior margin of the limb bud.
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| TS19: footplate mesenchyme |
Weak |
Regionally restricted |
6D
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A small focus of expression is sometimes seen in the anterior mesenchyme
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Analysis Tools
