Gene Expression Data
Assay Details
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Assay
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Reference:
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J:43752
Xiang M, et al., Requirement for Brn-3c in maturation and survival, but not in fate determination of inner ear hair cells. Development. 1998 Oct;125(20):3935-46
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:1289491
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Gene symbol:
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Pou4f3
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Gene name:
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POU domain, class 4, transcription factor 3
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Antibody:
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anti-Brn-3c
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
1A:
embryonic day 11.5
(more  )
Note: The plane of section was coronal.
Specimen
1A:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was coronal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: inner ear |
Absent |
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1A
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Expression was absent in the sensory epithelia of the otocyst.
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Specimen
1B:
embryonic day 12.5
(more )
Note: The plane of section was coronal.
Specimen
1B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was coronal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: inner ear |
Present |
Regionally restricted |
1B
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Expression was detected in scattered cells of the presumptive sensory epithelium (hair cells). Labeled cells in the supporting cell layer appear to be streaming towards the lumenal layer.
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Specimen
1C:
embryonic day 13.5
(more )
Note: The plane of section was coronal.
Specimen
1C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was coronal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inner ear vestibular component |
Present |
Regionally restricted |
1C
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Expression was detected in sensory epithelia of the otocyst including the cochlear and vestibular hair cells.
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Specimen
1D:
embryonic day 13.5
(more )
Note: The plane of section was coronal.
Specimen
1D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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The plane of section was coronal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inner ear vestibular component |
Present |
Regionally restricted |
1D
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Expression was detected in sensory epithelia of the otocyst including the cochlear and vestibular hair cells.
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| TS21: inner ear |
Present |
Regionally restricted |
1D
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Labeled cells in the supporting cell layer appear to be streaming towards the lumenal layer.
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Specimen
1E:
embryonic day 14.5
(more )
Note: The plane of section was coronal.
Specimen
1E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was coronal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
Regionally restricted |
1E
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Expression was detected in hair cells within the sensory organs including the organ of Corti.
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| TS22: inner ear vestibular component |
Present |
Regionally restricted |
1E
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Expression was detected in hair cells within the sensory organs including the maculae and cristae.
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Specimen
1F:
embryonic day 15.5
(more )
Note: The plane of section was coronal.
Specimen
1F:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was coronal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cochlea |
Present |
Regionally restricted |
1F
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Expression was detected in hair cells within the sensory organs including the organ of Corti.
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| TS23: inner ear vestibular component |
Present |
Regionally restricted |
1F
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Expression was detected in hair cells within the sensory organs including the maculae and cristae.
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Specimen
1G:
postnatal day 2
(more )
Note: The plane of section was coronal.
Specimen
1G:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was coronal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: inner ear |
Present |
Regionally restricted |
1G
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Expression was detected in hair cells within the sensory organs of the cochlea and the vestibular system including the maculae and cristae.
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Specimen
1H:
postnatal day 2
(more )
Note: The plane of section was coronal.
Specimen
1H:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was coronal.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: inner ear |
Present |
Regionally restricted |
1H
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Expression was detected in hair cells within the sensory organs including the organ of Corti.
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Specimen
1I:
postnatal adult
(more )
Note: The plane of section was coronal.
Specimen
1I:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The plane of section was coronal.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: inner ear |
Present |
Regionally restricted |
1I
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Expression was detected in hair cells within the sensory organs including the organ of Corti.
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Specimen
1J:
postnatal adult
(more )
Note: The plane of section was coronal.
Specimen
1J:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
|
Note:
|
The plane of section was coronal.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: inner ear |
Present |
Regionally restricted |
1J
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Expression was detected in hair cells within the sensory organs of the cochlea and the vestibular system including the maculae and cristae.
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Specimen
Not Shown:
embryonic day 17.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: inner ear |
Present |
Regionally restricted |
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Expression was detected in the hair cells of the lumenal layer. Also, labeled cells are present in the supporting cell layer that appear to be streaming towards the lumenal layer.
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Specimen
Not Shown:
embryonic day 19.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 19.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: inner ear |
Present |
Regionally restricted |
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Expression was detected in the hair cells of the lumenal layer. Also, labeled cells are present in the supporting cell layer that appear to be streaming towards the lumenal layer.
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Specimen
3A:
embryonic day 15.5
(more )
Note: Double labeled: blue - Pou4f3; red - Myo6 (assay MGI:2448677) .
Specimen
3A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Pou4f3; red - Myo6 (assay MGI:2448677) .
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: inner ear vestibular component |
Present |
Regionally restricted |
3A
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Expression was detected in the hair cells of the lumenal layer, staining colocalized with Myo6. Labeled cells in the supporting cell layer appear to be streaming towards the lumenal layer, staining did not colocalize with Myo6.
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Specimen
3C:
embryonic day 15.5
(more )
Note: Double labeled: blue - Pou4f3; red - Myo7a (assay MGI:2448681).
Specimen
3C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Pou4f3; red - Myo7a (assay MGI:2448681).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: inner ear vestibular component |
Present |
Regionally restricted |
3C
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Expression was detected in the hair cells of the lumenal layer, staining colocalized with Myo7a. Labeled cells in the supporting cell layer appear to be streaming towards the lumenal layer, staining did not colocalize with Myo7a.
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Specimen
3E:
postnatal day 0
(more )
Note: Double labeled: blue - Pou4f3; red - Myo7a (assay MGI:2448681).
Specimen
3E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Pou4f3; red - Myo7a (assay MGI:2448681).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: inner ear |
Present |
Regionally restricted |
3E
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Expression was detected in the hair cells of the lumenal layer, staining colocalized with Myo7a. Labeled cells in the supporting cell layer appear to be streaming towards the lumenal layer, staining did not colocalize with Myo7a.
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Specimen
Not Shown:
embryonic day 13.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: inner ear vestibular component |
Present |
Single cells |
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Expression was detected in the hair cells of the lumenal layer. Labeled cells in the supporting cell layer appear to be streaming towards the lumenal layer.
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