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Gene Expression Data
Assay Details
Assay
Reference: J:46439 Freeman TC, et al., Expression Mapping of Mouse Genes. MGI Direct Data Submission. 1998;
Assay type: RT-PCR
MGI Accession ID: MGI:1204378
Gene symbol: Wnt5a
Gene name: wingless-type MMTV integration site family, member 5A
Probe: st96_142
Visualized with: Ethidium bromide
Assay notes: MgCl2: 3.5 mM; Annealing temp: 55C; Annealing time: 90sec; Cycles: x50
Results
Image: st96_142 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 189 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
Lane 1 P w 6-8 TS28: brain Strong 0.05 µg; total RNA C57BL/6J Pooled
Lane 2 P w 6-8 TS28: cerebral cortex Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 3 P w 6-8 TS28: hippocampus Absent 0.05 µg; total RNA C57BL/6J Pooled
Lane 4 P w 6-8 TS28: midbrain Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 5 P w 6-8 TS28: corpus striatum Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 6 P w 6-8 TS28: main olfactory bulb Strong 0.05 µg; total RNA C57BL/6J Pooled
Lane 7 P w 6-8 TS28: cerebellum Strong 0.05 µg; total RNA C57BL/6J Pooled
Lane 8 P w 6-8 TS28: spinal cord Strong 0.05 µg; total RNA C57BL/6J Pooled
Lane 9 P w 6-8 TS28: pituitary gland Absent 0.05 µg; total RNA C57BL/6J Pooled
Lane 10 P w 6-8 TS28: eye Strong 0.05 µg; total RNA C57BL/6J Pooled
Lane 11 P w 6-8 TS28: skeletal muscle Trace 0.05 µg; total RNA C57BL/6J Pooled
Lane 12 P w 6-8 TS28: heart Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 13 P w 6-8 TS28: lung Absent 0.05 µg; total RNA C57BL/6J Pooled
Lane 14 P w 6-8 TS28: spleen Trace 0.05 µg; total RNA C57BL/6J Pooled
Lane 15 P w 6-8 TS28: liver Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 16 P w 6-8 TS28: gallbladder Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 17 P w 6-8 TS28: pancreas Absent 0.05 µg; total RNA C57BL/6J Pooled
Lane 18 P w 6-8 TS28: metanephros Absent 0.05 µg; total RNA C57BL/6J Pooled
Lane 19 P w 6-8 TS28: bladder Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 20 P w 6-8 TS28: adrenal gland Strong 0.05 µg; total RNA C57BL/6J Pooled
Lane 21 P w 6-8 TS28: skin Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 22 P w 6-8 TS28: adipose tissue Strong 0.05 µg; total RNA C57BL/6J Pooled
Lane 23 P w 6-8 TS28: femur Absent 0.05 µg; total RNA C57BL/6J Pooled
Lane 24 P w 6-8 TS28: hand Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 25 P w 6-8 TS28: incisor Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 26 P w 6-8 TS28: tongue Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 27 P w 6-8 TS28: salivary gland Absent 0.05 µg; total RNA C57BL/6J Pooled
Lane 28 P w 6-8 TS28: esophagus Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 29 P w 6-8 TS28: stomach fundus Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 30 P w 6-8 TS28: pyloric antrum Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 31 P w 6-8 TS28: duodenum Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 32 P w 6-8 TS28: jejunum Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 33 P w 6-8 TS28: ileum Strong 0.05 µg; total RNA C57BL/6J Pooled
Lane 34 P w 6-8 TS28: cecum Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 35 P w 6-8 TS28: ascending colon Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 36 P w 6-8 TS28: descending colon Moderate 0.05 µg; total RNA C57BL/6J Pooled
Lane 37 P w 6-8 TS28: apex of cecum Strong 0.05 µg; total RNA C57BL/6J Pooled
Lane 38 P w 6-8 TS28: thymus Trace 0.05 µg; total RNA C57BL/6J Pooled
Lane 39 P w 6-8 TS28: mammary gland Moderate 0.05 µg; total RNA C57BL/6J Female Non-pregnant
Lane 40 P w 6-8 TS28: mammary gland Strong 0.05 µg; total RNA C57BL/6J Female Pregnant
Lane 41 P w 6-8 TS28: testis Moderate 0.05 µg; total RNA C57BL/6J Male
Lane 42 P w 6-8 TS28: epididymis Strong 0.05 µg; total RNA C57BL/6J Male
Lane 43 P w 6-8 TS28: ovary Trace 0.05 µg; total RNA C57BL/6J Female
Lane 44 P w 6-8 TS28: cervix uteri Trace 0.05 µg; total RNA C57BL/6J Female
Lane 45 P w 6-8 TS28: placenta Strong 0.05 µg; total RNA C57BL/6J Female
Lane 46 E15-16 TS23: embryo Strong 0.05 µg; total RNA C57BL/6J Pooled
Lane 47 Control
Lane 48 Control
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory