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Fostm2.1(icre/ERT2)Luo - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Fostm2.1(icre/ERT2)Luo
FBJ osteosarcoma oncogene; targeted mutation 2.1, Liqun Luo 		Driver: Fos
Type: Targeted (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: Fos2A-iCreER, Fos2A-iCreERT2, TRAP2
Molecular description: A targeting vector was designed to insert a 2A::iCreER::frt-neo-frt cassette into the translational stop site of the FBJ osteosarcoma oncogene locus (Fos) on chromosome 12. The viral 2A oligopeptide sequence mediates ribosomal skipping. The iCreER fusion gene used here has an improved/optimized variant of Cre recombinase (iCre) that is fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain (ER). TThe targeting construct was electroporated into 129;FVB-derived embryonic stem (ES) cells, and correctly targeted ES cells were injected into recipient blastocysts. Chimeric animals were bred to CD1 mice and/or germline-active GFP-FlpO transgenic mice (mixed genetic background including CD1) to delete the frt-flanked neo cassette.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Fostm2.1(icre/ERT2)Luo activity also observed in:
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Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 54 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
05/14/2024
MGI 6.23 		The Jackson Laboratory