Pdgfrb<tm1.1(cre/ERT2)Csln> - Nervous System Recombinase Activity Detail MGI Mouse

Pdgfrb^{tm1.1(cre/ERT2)Csln} - Nervous System
Recombinase Activity Detail

Allele Information | Tissue Information | Recombinase Activity | References

Allele Information

Allele:

Pdgfrb^{tm1.1(cre/ERT2)Csln}
platelet derived growth factor receptor, beta polypeptide; targeted mutation 1.1, Chyuan-Sheng Lin

Driver: Pdgfrb
Type: Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Synonym:

PDGFRbata-P2A-CreERT2

Molecular description:

A targeting vector was designed to replace the translation stop codon of the platelet derived growth factor receptor, beta polypeptide (Pdgfrb) gene with a creER fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain) followed by a frt-flanked neomycin resistance (neo) cassette. A viral P2A oligopeptide, from porcine teschovirus-1, was inserted upstream of the creER fusion gene to ribosomal skipping. The construct was electroporated into (C57BL/6J x 129S6/SvEvTac)F1-derived KV1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL6/N blastocysts and resulting chimeric males were bred to Tg(ACTFLPe)9205Dym/J females to remove the neo cassette. These mice express Cre-ER protein in pericytes in the vasculature of organs including brain and retina. Restricted to the cytoplasm, Cre-ER can only gain access to the nuclear compartment after exposure to tamoxifen.

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Pdgfrb^{tm1.1(cre/ERT2)Csln} activity also observed in:
cardiovascular system, head, muscle, sensory organs

Tissue Information

Nervous System

Other recombinase alleles with activity in Nervous System tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acsm4^tm1(cre)Hsak, Adcyap1r1^{tm1.1(cre/GFP)Rpa} ...(more)

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

16 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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