Pvalb<tm1.1(cre/ERT2)Hze> - Head Recombinase Activity Detail MGI Mouse

Pvalb^{tm1.1(cre/ERT2)Hze} - Head
Recombinase Activity Detail

Allele Information | Tissue Information | Recombinase Activity | References

Allele Information

Allele:

Pvalb^{tm1.1(cre/ERT2)Hze}
parvalbumin; targeted mutation 1.1, Hongkui Zeng

Driver: Pvalb
Type: Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Synonym:

Pvalb-2A-CreERT2, Pvalb-2A-CreERT2-Deltahygro, Pvalb^{tm1.1(cre/ERT2)Htz}

Molecular description:

Embryonic stem (ES) cells, previously targeted with a T2A-Cre vector immediately downstream of the parvalbumin translational STOP codon were re-targeted with a T2A-CreERT2 vector and a FLP-expressing plasmid to facilitate recombination. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a CreERT2 fusion gene, the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette and an attP site. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the attB/attP-flanked PGK cassette. The resulting mice were bred with C57BL/6J wildtype mice for at least two additional generations (and the PhiC31 transgene was removed).

Find mice (IMSR):

Mouse Strains: 1 strain available Cell Lines: 0 lines available

Additional Tissues:

Pvalb^{tm1.1(cre/ERT2)Hze} activity also observed in:
nervous system

Tissue Information

Head

Other recombinase alleles with activity in Head tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acan^{tm1(cre/ERT2)Crm}, Acsm4^tm1(cre)Hsak ...(more)

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

7 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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