About   Help   FAQ
Tg(Nes-phiC31*/ERT2)2Imayo - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Nes-phiC31*/ERT2)2Imayo
transgene insertion 2, Itaru Imayoshi 		Driver: Nes
Type: Transgenic (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: Nestin-PhiC31-ERT2 line 2
Molecular description: The construct was composed of a 5.8 kb fragment of the nestin promoter with sequences for optimized phiC31 recombinase (phiC31*; coding sequence in this construct lacks the nuclear localization signal) fused to the tamoxifen-inducible mutated estrogen receptor sequence (ERT2), followed by the 1.8 kb nestin second intron containing the neural stem/progenitor cell-specific enhancer. The SV40 polyadenylation sequence was also cloned into the vector. The purified construct was injected into fertilized one-cell embryos. 7 founders were established with 2 lines (#2 and 4) chosen for further analysis based on high transgene expression in the ventricular zone of the developing nervous system.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(Nes-phiC31*/ERT2)2Imayo activity also observed in:
head
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Recombinase Activity
Click heading to re-sort table.
Assays Genotypic Background Result Notes  
References
All for this allele: 1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/14/2024
MGI 6.23 		The Jackson Laboratory