About   Help   FAQ
Tg(Pmch-cre)1Lowl - Head
Recombinase Activity Detail
Allele Information
Allele:
Tg(Pmch-cre)1Lowl
transgene insertion 1, Bradford B Lowell 		Driver: Pmch
Type: Transgenic (Recombinase)
Synonym: MCH-Cre
Molecular description: Homologous recombination with EL250 cells was induced to insert a cre-polyA-FRT-Neo-FRT cassette into a BAC containing the Pmch (pro-melanin-concentrating hormone; MCH) at the Pmch translational start site with concomitant replacement of the 26 nucleotides following it. Arabinose-induced Flp recombination in bacterial cells removed the kanamycin cassette. The purified, linearized construct was injected into the pronuclei of FVB one-cell embryos. Eight lines were generated and crossed to Z/EG reporter animals. 2 lines exhibited GFP expression in the lateral hypothalamus and zona incerta, known sites of endogenous Pmch expression. Cre expression in one of these lines of characterized further.
Find mice (IMSR): Mouse Strains: 2 strains available       Cell Lines: 0 lines available
Additional Tissues: Tg(Pmch-cre)1Lowl activity also observed in:
nervous system
Tissue Information
Head Other recombinase alleles with activity in Head tissues:
Recombinase Activity
Click heading to re-sort table.
Assays Genotypic Background Result Notes  
References
All for this allele: 28 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/14/2024
MGI 6.23 		The Jackson Laboratory