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Tg(BEST1-rtTA,tetO-cre)1Yzl - Head
Recombinase Activity Detail
Allele Information
Allele:
Tg(BEST1-rtTA,tetO-cre)1Yzl
transgene insertion 1, Yun-Zheng Le 		Driver: BEST1
Type: Transgenic (Inducible, Recombinase, Transactivator)
Inducer: doxycycline/tetracycline
Synonym: PVMD2-rtTA::tetO-PhCMVcre, VMD2-cre
Molecular description: Two transgenic fragments were co-injected to generate the mouse line. One fragment contains a 3 kb human BEST1 promoter fragment driving expression of the tetracycline-inducible transactivator rtTA and the SV40 polyA sequence. The second fragment consists of the tetracycline-responsive regulatory element (tetO) and human cytomegalovirus immediate early enhancer (hCMV) controlling expression of a translationally optimized cre sequence followed by the mouse metallothionein (Mt1) polyadenylation signal. After co-injection into zygotes, 10 founders were obtained with co-integration of both fragments into a single chromosome being observed in all founders. One line demonstrating substantial activity in Muller glial cells was characterized further.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(BEST1-rtTA,tetO-cre)1Yzl activity also observed in:
sensory organs
Tissue Information
Head Other recombinase alleles with activity in Head tissues:
Images
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J:287362 Fig. 2
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 40 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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05/14/2024
MGI 6.23 		The Jackson Laboratory