Clone 9 Probe Detail MGI Mouse MGI:15354

Clone 9 Probe Detail

Name

Clone 9
Sequence Type

cDNA
ID

MGI:15354
Region Covered

most of coding region and 3' untranslated sequence
Child Clone

Prn-p cDNA
Vector Type

Phage
Insert Site

EcoRI
Insert Size

1.7 kb
Note

Clone 9 consists of a 0.8 kb fragment (subclone 9-9) and a 0.9 kb fragment (subclone 9-13) joined at an EcoRI site. Subclone 9-13 is also referred to as cDNA fragment number 13. The insert from Clone 9 was subcloned into pEMBL8.

Species

mouse, laboratory
Strain

Swiss
Tissue

brain
Tissue Description

scrapie infected brain

Prnp

prion protein

M30384 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)

J:8918 Westaway D, et al., Cell. 1987 Nov 20;51(4):651-62

Notes: A 0.9 kb fragment (cDNA fragment 13) derived from Clone 9 was used for Southern analysis. Additional restiction site polymorphisms between strains NZW and I/Ln (XbaI, PstI, and EcoRI) lie 3' to exon 2.

Endonuclease	Gene	Allele	Fragments	Strains
BstEII	Prnp	a	5.7,3.5kb	NZW
BstEII	Prnp	b	15.5kb	I/Ln, IM, P/J

J:7876 Chesebro B, et al., Identification of scrapie prion protein-specific mRNA in scrapie-infected and uninfected brain. Nature. 1985 May 23-29;315(6017):331-3

J:8406 Locht C, et al., Molecular cloning and complete sequence of prion protein cDNA from mouse brain infected with the scrapie agent. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6372-6

J:8918 Westaway D, et al., Distinct prion proteins in short and long scrapie incubation period mice. Cell. 1987 Nov 20;51(4):651-62

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