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primer 5, primer 6 Primer Detail
Primers
  • Name
    primer 5, primer 6
  • Primer 1 Sequence
    CCTTCAATGGATCCCAGTCCAAGGAGGAGG
  • Primer 2 Sequence
    GTTCTAGACTCAAGCTTCATCTGTGTG
  • ID
    MGI:138
  • Product Size
    888bp
Genes
Vwf Von Willebrand factor
Polymorphisms
J:12631 Barrow LL, et al., Mamm Genome. 1993;4(6):343-5
Endonuclease Gene Allele Fragments Strains
Vwf b ~590bp C57BL/6J
s ~400bp SPRET/EiJ
J:18453 Nichols WC, et al., Blood. 1994 Jun 1;83(11):3225-31
Notes: PCR amplified fragment was digested with RsaI, fractionated on a 4% agarose gel, and visualized after staining with ethidium bromide.
Endonuclease Gene Allele Fragments Strains
RsaI Vwf a 426,214,127,99,22bp RIIIS/J
b 240,214,186,127,99,22bp PWK/PhJ
References
J:12631 Barrow LL, et al., Conserved linkage of neurotrophin-3 and von Willebrand factor on mouse chromosome 6. Mamm Genome. 1993;4(6):343-5
J:18453 Nichols WC, et al., von Willebrand disease in the RIIIS/J mouse is caused by a defect outside of the von Willebrand factor gene [published erratum appears in Blood 1995 Sep 15;86(6):2461]. Blood. 1994 Jun 1;83(11):3225-31
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory