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F5, R5 Primer Detail
Primers
  • Name
    F5, R5
  • Primer 1 Sequence
    TCCAACGCAGCTGAGACCGTGATAG
  • Primer 2 Sequence
    GTAACCCACATGCATGCAGTACCTG
  • ID
    MGI:132
  • Product Size
    200-585bp
  • Note
    Amplifies a B2 repeat in exon 10.
Genes
Phkg1 phosphorylase kinase gamma 1
Polymorphisms
J:4391 Maichele AJ, et al., Genomics. 1993 Apr;16(1):139-49
Notes: Genomic DNA was amplified via PCR, electrophoresed on a 4% agarose gel and viewed with ethidium bromide.
Endonuclease Gene Allele Fragments Strains
Phkg1 a 585.0bp BALB/c, M. m. molossinus
b 200.0bp AT29, C3H, C57BL/10, I/LnJ, ICR, M. m. domesticus, M. spretus, YBR
c 565.0kb M. m. castaneus
J:16406 Maichele AJ, et al., Mamm Genome. 1994 Jan;5(1):15-8
Notes: Products were amplified by PCR then separated on a 6% denaturing polyacrylamide gel.
Endonuclease Gene Allele Fragments Strains
Phkg1 a present A/J, BALB/c, CASA/RkJ, DF, M. m. domesticus poschiavinus, MOLD/RkJ
b absent AKR/J, C3H/HeJ, C57BL/6J, C57BL/10, C57L/J, DBA/2J, M. m. musculus, M. spretus, WSB
References
J:4391 Maichele AJ, et al., A B2 repeat insertion generates alternate structures of the mouse muscle gamma-phosphorylase kinase gene. Genomics. 1993 Apr;16(1):139-49
J:16406 Maichele AJ, et al., The gamma phosphorylase kinase gene, Phkg, maps to mouse chromosome 5 near Gus. Mamm Genome. 1994 Jan;5(1):15-8
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory