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pSAM-1 Probe Detail
Nucleotide
Probe/Clone
  • Name
    pSAM-1
  • Sequence Type
    genomic
  • ID
    MGI:12317
  • Region Covered
    encodes the functional Aprt gene, exons 1-5
  • Parent Clone
    pMaprt-11
  • Vector Type
    Plasmid
  • Insert Site
    EcoRI/SphI
  • Insert Size
    3.1 kb
  • Synonyms
    pSAM3.1
Source
  • Species
    mouse, laboratory
  • Strain
    BALB/c
  • Sex
    Male
  • Tissue
    sperm
Genes
Aprt adenine phosphoribosyl transferase
Aprt-ps adenine phosphoribosyl transferase, pseudogene
Polymorphisms
J:8422 Farber RA, et al., Cytogenet Cell Genet. 1986;42(4):198-201
Notes: The polymorphic fragments 6.1 kb (C57BL/6J) and 3.2 kb (AKR/J) result from a RFLV in the Aprt-ps on chromosome 8. The 7.9 kb band contains the functional Aprt gene and the remaining bands are thought to represent other pseudogenes.
Endonuclease Gene Allele Fragments Strains
BglII Aprt-ps a 7.9,5.9,4.9,3.2kb AKR/J
b 7.9,6.1,5.9,4.9kb C57BL/6J
J:3742 Abonia JP, et al., Mamm Genome. 1993;4(1):25-32
Notes: A 3.1kb EcoRI/SalI fragment was used for hybridization.
Endonuclease Gene Allele Fragments Strains
BglI Aprt a 2.7,2.5kb BALB/cByJ, C3H/HeJ, C57BL/6J
m 4.2,2.7kb PWK
KpnI Aprt a 5.3kb BALB/cByJ, C3H/HeJ, C57BL/6J
t 6.8,5.4kb STS/A
References
J:7132 Sikela JM, et al., Cloning and expression of a mouse adenine phosphoribosyltransferase gene. Gene. 1983 May-Jun;22(2-3):219-28
J:7839 Dush MK, et al., Nucleotide sequence and organization of the mouse adenine phosphoribosyltransferase gene: presence of a coding region common to animal and bacterial phosphoribosyltransferases that has a variable intron/exon arrangement. Proc Natl Acad Sci U S A. 1985 May;82(9):2731-5
J:8422 Farber RA, et al., Assignment of a processed mouse Aprt pseudogene to the same chromosome as the functional gene. Cytogenet Cell Genet. 1986;42(4):198-201
J:8539 Dush MK, et al., An unusual adenine phosphoribosyltransferase pseudogene is syntenic with its functional gene and is flanked by highly polymorphic DNAs. Mol Cell Biol. 1986 Dec;6(12):4161-7
J:3742 Abonia JP, et al., Linkage of Agt and Actsk-1 to distal mouse chromosome 8 loci: a new conserved linkage. Mamm Genome. 1993;4(1):25-32
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory