pSAM-1 Probe Detail MGI Mouse MGI:12317

pSAM-1 Probe Detail

Name

pSAM-1
Sequence Type

genomic
ID

MGI:12317
Region Covered

encodes the functional Aprt gene, exons 1-5
Parent Clone

pMaprt-11
Vector Type

Plasmid
Insert Site

EcoRI/SphI
Insert Size

3.1 kb
Synonyms

pSAM3.1

Species

mouse, laboratory
Strain

BALB/c
Sex

Male
Tissue

sperm

Aprt	adenine phosphoribosyl transferase
Aprt-ps	adenine phosphoribosyl transferase, pseudogene

J:8422 Farber RA, et al., Cytogenet Cell Genet. 1986;42(4):198-201

Notes: The polymorphic fragments 6.1 kb (C57BL/6J) and 3.2 kb (AKR/J) result from a RFLV in the Aprt-ps on chromosome 8. The 7.9 kb band contains the functional Aprt gene and the remaining bands are thought to represent other pseudogenes.

Endonuclease	Gene	Allele	Fragments	Strains
BglII	Aprt-ps	a	7.9,5.9,4.9,3.2kb	AKR/J
BglII	Aprt-ps	b	7.9,6.1,5.9,4.9kb	C57BL/6J

J:3742 Abonia JP, et al., Mamm Genome. 1993;4(1):25-32

Notes: A 3.1kb EcoRI/SalI fragment was used for hybridization.

Endonuclease	Gene	Allele	Fragments	Strains
BglI	Aprt	a	2.7,2.5kb	BALB/cByJ, C3H/HeJ, C57BL/6J
BglI	Aprt	m	4.2,2.7kb	PWK
KpnI	Aprt	a	5.3kb	BALB/cByJ, C3H/HeJ, C57BL/6J
KpnI	Aprt	t	6.8,5.4kb	STS/A

J:7132 Sikela JM, et al., Cloning and expression of a mouse adenine phosphoribosyltransferase gene. Gene. 1983 May-Jun;22(2-3):219-28

J:7839 Dush MK, et al., Nucleotide sequence and organization of the mouse adenine phosphoribosyltransferase gene: presence of a coding region common to animal and bacterial phosphoribosyltransferases that has a variable intron/exon arrangement. Proc Natl Acad Sci U S A. 1985 May;82(9):2731-5

J:8422 Farber RA, et al., Assignment of a processed mouse Aprt pseudogene to the same chromosome as the functional gene. Cytogenet Cell Genet. 1986;42(4):198-201

J:8539 Dush MK, et al., An unusual adenine phosphoribosyltransferase pseudogene is syntenic with its functional gene and is flanked by highly polymorphic DNAs. Mol Cell Biol. 1986 Dec;6(12):4161-7

J:3742 Abonia JP, et al., Linkage of Agt and Actsk-1 to distal mouse chromosome 8 loci: a new conserved linkage. Mamm Genome. 1993;4(1):25-32

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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