IV92 Probe Detail MGI Mouse MGI:12220

IV92 Probe Detail

Name

IV92
Sequence Type

cDNA
ID

MGI:12220
Region Covered

encodes an Igh-VJ558 gene segment, Igh-D, Igh-J segments and the C1 exon of the Igh-4 gene segment
Vector Type

Plasmid
Insert Site

PstI
Insert Size

600 bp

Species

mouse, laboratory
Cell Line

hybridoma cell line G8Ca1.7

Ighg1	immunoglobulin heavy constant gamma 1 (G1m marker)
Igh-D	immunoglobulin heavy chain diversity region
Igh-J	immunoglobulin heavy chain, joining region
Igh-VJ558	immunoglobulin heavy chain (J558 family)

J:7120 Schiff C, et al., Nucleic Acids Res. 1983 Jun 25;11(12):4007-17

Notes: A PstI fragment of ~230 bp derived from the variable region of IV92 was isolated and used in Southern analysis.

Endonuclease	Gene	Allele	Fragments	Strains
BglII	Igh-VJ558	a	20,12,8.5,5.5,4.2,3.9kb	BALB/c
		b	13,11.5,9,6.5,5.3,4.2, 3.9kb	C57BL/6
		c	15,10.5,8.5,5.5,4.2,3.9kb	DBA/2
EcoRI	Igh-VJ558	a	7.5,7.0,4.3,3.0kb	BALB/c, DBA/2
EcoRI	Igh-VJ558	b	9.4,7.2,4.5kb	C57BL/6

J:7120 Schiff C, et al., Immunoglobulin diversity: analysis of the germ-line VH gene repertoire of the murine anti-GAT response. Nucleic Acids Res. 1983 Jun 25;11(12):4007-17

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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