pAF81 Probe Detail MGI Mouse MGI:12151

pAF81 Probe Detail

Name

pAF81
Sequence Type

cDNA
ID

MGI:12151
Region Covered

most of the coding region and a brief stretch (14 bp) of 3' untranslated sequence
Child Clone

pAF81-3'
Vector Type

Plasmid
Insert Site

PstI
Insert Size

1.1 kb

Species

mouse, laboratory
Strain

BALB/c
Age

postnatal day 10.0
Tissue

skeletal muscle

Actc1

actin, alpha, cardiac muscle 1

M10652 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)

X03767 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)

J:7113 Minty AJ, et al., J Mol Biol. 1983 Jun 15;167(1):77-101

Endonuclease	Gene	Allele	Fragments	Strains
BclI	Actc1	a	>23kb	BALB/c
BclI	Actc1	b	~20kb	C57BL/6

J:20290 Garner I, et al., EMBO J. 1986 Oct;5(10):2559-67

Notes: A 5' PstI-BglII fragment isolated from pAF81 (pAF81-5') was used for Southern analysis.

Endonuclease	Gene	Allele	Fragments	Strains
BclI	Actc1	a	23kb	BALB/c
BclI	Actc1	b	15kb	C3H
EcoRI	Actc1	a	5.3,9kb	BALB/c
EcoRI	Actc1	b	5.3kb	C3H
HindIII	Actc1	a	10,9.5kb	BALB/c
HindIII	Actc1	b	10kb	C3H

J:7113 Minty AJ, et al., Number and organization of actin-related sequences in the mouse genome. J Mol Biol. 1983 Jun 15;167(1):77-101

J:8272 Alonso S, et al., Comparison of three actin-coding sequences in the mouse; evolutionary relationships between the actin genes of warm-blooded vertebrates. J Mol Evol. 1986;23(1):11-22

J:20290 Garner I, et al., A 5' duplication of the alpha-cardiac actin gene in BALB/c mice is associated with abnormal levels of alpha-cardiac and alpha-skeletal actin mRNAs in adult cardiac tissue. EMBO J. 1986 Oct;5(10):2559-67

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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