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pGLC450 Probe Detail
Nucleotide
Probe/Clone
  • Name
    pGLC450
  • Sequence Type
    genomic
  • ID
    MGI:12075
  • Region Covered
    encodes an intronless Mylf pseudogene
  • Parent Clone
    TypeA phage
  • Child Clone
    pGLC450-1
  • Vector Type
    Plasmid
  • Insert Site
    BamHI
  • Insert Size
    470 bp
  • Note
    Probe name is not specified in many references; confirmed by personal communication.
Source
  • Species
    mouse, laboratory
  • Strain
    BALB/c
  • Sex
    Male
  • Tissue
    sperm
Genes
Myl4 myosin, light polypeptide 4
Myl3 myosin, light polypeptide 3
Myl1 myosin, light polypeptide 1
Mylf-ps myosin light chain, alkali, fast skeletal muscle, pseudogene
Sequences
K02243 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
Polymorphisms
J:7607 Robert B, et al., Cell. 1984 Nov;39(1):129-40
Endonuclease Gene Allele Fragments Strains
BamHI Mylf-ps b ~6.6kb BALB/c
s absent M. spretus
EcoRI Myl1 d 8.5,4.2kb DBA/2
s 6.5,4.2kb M. spretus
EcoRI Mylf-ps b ~5.5kb BALB/c
s absent M. spretus
J:7759 Robert B, et al., Nature. 1985 Mar 14-20;314(6007):181-3
Notes: Allele symbols assigned.
Endonuclease Gene Allele Fragments Strains
BclI Myl1 d not given DBA/2
s not given SPE/Pas
EcoRI Myl1 d not given DBA/2
s not given SPE/Pas
PstI Myl1 b not given C57BL/6J
d not given DBA/2J
References
J:7607 Robert B, et al., A single locus in the mouse encodes both myosin light chains 1 and 3, a second locus corresponds to a related pseudogene. Cell. 1984 Nov;39(1):129-40
J:7759 Robert B, et al., Investigation of genetic linkage between myosin and actin genes using an interspecific mouse back-cross. Nature. 1985 Mar 14-20;314(6007):181-3
J:7902 Barton PJ, et al., The myosin alkali light chains of mouse ventricular and slow skeletal muscle are indistinguishable and are encoded by the same gene. J Biol Chem. 1985 Jul 15;260(14):8578-84
J:8102 Barton PJ, et al., The same myosin alkali light chain gene is expressed in adult cardiac atria and in fetal skeletal muscle. J Muscle Res Cell Motil. 1985 Aug;6(4):461-75
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/30/2025
MGI 6.24 		The Jackson Laboratory