pH-2III Probe Detail MGI Mouse MGI:12066

pH-2III Probe Detail

Name

pH-2III
Sequence Type

cDNA
ID

MGI:12066
Region Covered

hybridizes to exons 2 and 3 of Class I genes
Vector Type

Plasmid
Insert Site

PstI
Insert Size

300bp
Note

Hybridizes to all class I sequences.

Species

mouse, laboratory
Strain

BALB/c
Tissue

lymphocyte
Cell Line

lymphoma cell line RDM-4

H2	histocompatibility-2, MHC
H2-D1	histocompatibility 2, D region locus 1

J:20105 Steinmetz M, et al., Cell. 1981 Apr;24(1):125-34

Notes: A 287 bp PstI fragment was isolated from pH-2III and used in Southern analysis.

Endonuclease	Gene	Allele	Fragments	Strains
BamHI	H2-D1	d	6.2kb	BALB/c
BamHI	H2-D1	k	5.4kb	CBA

J:6934 Shin HS, et al., Cell. 1982 Jul;29(3):969-76

Notes: A 350 bp PstI fragment was isolated from pH-2III and used in Southern analysis. Strains and/or t haplotype designations appear in the allele groupings.

Endonuclease	Gene	Allele	Fragments	Strains
BamHI	H2	a	multiple	BALB/c
		b	multiple	AKR, C3H/DiSn, C57BL/6-H2^k
		c	multiple	C3.t¹²/t¹, C3.t^w12/t^w32, C3H-t^w2
		d	multiple	B6.K1
		e	multiple	B6.TL
		f	multiple	A/J
		g	multiple	SM/J
		h	multiple	SWR/J
EcoRI	H2	a	multiple	BALB/c, C3H-t^w71
		b	multiple	AKR, C3.t^w75, C3H/DiSn, C57BL/6-H2^k
		c	multiple	C3.t¹²/t¹, C3.t^w5, C3.t^w12, C3H-t^w2, STOCK t^w32
		d	multiple	B6.K1, STOCK t^w32
		e	multiple	B6.TL, C3.t^w12
		f	multiple	A/J, C3.t⁰
		g	multiple	C3H/DiSn, SM/J
		h	multiple	SWR/J

J:7253 Shin HS, et al., Nature. 1983 Nov 24-30;306(5941):380-3

Notes: t haplotype designations, rather than strain designations appear in the allele groupings.

Endonuclease	Gene	Allele	Fragments	Strains
EcoRI	H2-D1	a	23kb	C3.t^w12
EcoRI	H2-D1	b	21kb	STOCK t^w32

J:7675 Shin HS, et al., Cell. 1984 Dec;39(3 Pt 2):573-8

Notes: Strain and/or t haplotype designations appear in the allele groupings.

Endonuclease	Gene	Allele	Fragments	Strains
HindIII	H2-D1	a	9.5kb	C3.t¹², STOCK t^w32
		b	9.0kb	C3H-T/t^w5, C3H-T/t^w12
		c	5.8kb	C3.t^w5

J:11962 Steinmetz M, et al., A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell. 1981 Sep;25(3):683-92

J:20105 Steinmetz M, et al., Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes. Cell. 1981 Apr;24(1):125-34

J:6934 Shin HS, et al., Genetic structure and origin of t haplotypes of mice, analyzed with H-2 cDNA probes. Cell. 1982 Jul;29(3):969-76

J:7253 Shin HS, et al., Inversion in the H-2 complex of t-haplotypes in mice. Nature. 1983 Nov 24-30;306(5941):380-3

J:7675 Shin HS, et al., Gene mapping within the T/t complex of the mouse. IV: The inverted MHC is intermingled with several t-lethal genes. Cell. 1984 Dec;39(3 Pt 2):573-8

J:7933 Lader E, et al., Definitive chromosomal location of the H-2 complex by in situ hybridization to pachytene chromosomes. Immunogenetics. 1985;22(1):49-54

J:8713 Uehara H, et al., The molecular organization of the H-2K region of two t-haplotypes: implications for the evolution of genetic diversity. EMBO J. 1987 Jan;6(1):83-90

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