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pMCAII Probe Detail
Nucleotide
Probe/Clone
  • Name
    pMCAII
  • Sequence Type
    cDNA
  • ID
    MGI:11991
  • Region Covered
    pMCAII encodes all of the 3' noncoding sequence and most of the coding region
  • Vector Type
    Plasmid
  • Insert Site
    PstI
  • Insert Size
    ~1500 bp
  • Note
    pMCAII is a Car-2 cDNA that cross hybridizes with Car-1.
  • Synonyms
    p6-69
Source
  • Species
    mouse, laboratory
  • Strain
    BALB/c
  • Tissue
    spleen
  • Tissue Description
    anemic spleen
Genes
Car2 carbonic anhydrase 2
Sequences
K00811 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
K00812 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
M11830 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
M11830 (GenBank | EMBL | ENA | DDBJ | MGI Sequence Detail)
Polymorphisms
J:8376 Bishop TR, et al., Proc Natl Acad Sci U S A. 1986 Aug;83(15):5568-72
Endonuclease Gene Allele Fragments Strains
RsaI Car2 b ~3.2,1.3,0.9kb AKR, C57BL/6, C58
d 3.4,1.3,0.9kb DBA/2, SM
J:10139 Moseley WS, et al., Genomics. 1989 Nov;5(4):899-905
Notes: A 450bp PstI fragment was used for hybridization.
Endonuclease Gene Allele Fragments Strains
BamHI Car2 c absent C3H/HeJ-Faslgld/J
s ~6.3kb M. spretus
References
J:7016 Curtis PJ, Cloning of mouse carbonic anhydrase mRNA and its induction in mouse erythroleukemic cells. J Biol Chem. 1983 Apr 10;258(7):4459-63
J:7299 Curtis PJ, et al., The nucleotide sequence and derived amino acid sequence of cDNA coding for mouse carbonic anhydrase II. Gene. 1983 Nov;25(2-3):325-32
J:8376 Bishop TR, et al., Isolation of a rat liver delta-aminolevulinate dehydrase (ALAD) cDNA clone: evidence for unequal ALAD gene dosage among inbred mouse strains. Proc Natl Acad Sci U S A. 1986 Aug;83(15):5568-72
J:8635 Fraser PJ, et al., Molecular evolution of the carbonic anhydrase genes: calculation of divergence time for mouse carbonic anhydrase I and II. J Mol Evol. 1986;23(4):294-9
J:10139 Moseley WS, et al., Definition of mouse chromosome 1 and 3 gene linkage groups that are conserved on human chromosome 1: evidence that a conserved linkage group spans the centromere of human chromosome 1. Genomics. 1989 Nov;5(4):899-905
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory