pDO-beta-2 Probe Detail MGI Mouse MGI:10296

pDO-beta-2 Probe Detail

Name

pDO-beta-2
Sequence Type

cDNA
ID

MGI:10296
Region Covered

encodes J[b]2.6, C[b]2 and 3' untranslated region
Child Clone

C-beta-2-specific probe
Insert Site

HindIII/EcoRI
Insert Size

~0.8 kb
Note

Hybridizes to C[b]1 and C[b]2 sequences.

Species

mouse, laboratory
Tissue

T-lymphocyte
Cell Line

hybridoma cell line DO.11.10

Tcrb	T cell receptor beta chain
Tcrb-C	T cell receptor beta, constant region
Tcrb-J	T cell receptor beta, joining region

J:7920 Kotzin BL, et al., Science. 1985 Jul 12;229(4709):167-71

Endonuclease	Gene	Allele	Fragments	Strains
BamHI	Tcrb	a	~8.0,~6.6kb	SJL
		c	~9.4,~6.6kb	BALB/c, NZB
		n	~8.0kb	NZW
EcoRI	Tcrb	a	9.4,~4kb	SJL
		c	9.4,~3kb	BALB/c, NZB
		n	9.4kb	NZW
HindIII	Tcrb	c	~9.4,~3.5kb	BALB/c, NZB, SJL
HindIII	Tcrb	n	~9.0kb	NZW
HpaII	Tcrb	a	9.4,6.6kb	SJL
		c	9.4,6.6kb	BALB/c, NZB
		n	~8.0kb	NZW
PvuII	Tcrb	a	~8.0,~11.0kb	SJL
		c	6.0,6.2kb	BALB/c, NZB
		n	5.0kb	NZW

J:8689 Kotzin BL, et al., J Exp Med. 1987 May 1;165(5):1237-51

Notes: The two NZB restriction fragments represent the C[b]1 and C[b]2 gene segments. The 5.0 kb NZW fragment represents C[b]2 only (NZW is deleted for C[b]1).

Endonuclease	Gene	Allele	Fragments	Strains
PvuII	Tcrb	c	6.0,6.2kb	NZB
PvuII	Tcrb	n	5.0kb	NZW

J:7843 Born W, et al., Rearrangement of T-cell receptor beta-chain genes during T-cell development. Proc Natl Acad Sci U S A. 1985 May;82(9):2925-9

J:7920 Kotzin BL, et al., A large deletion within the T-cell receptor beta-chain gene complex in New Zealand white mice. Science. 1985 Jul 12;229(4709):167-71

J:8689 Kotzin BL, et al., The contribution of NZW genes to lupus-like disease in (NZB x NZW)F1 mice. J Exp Med. 1987 May 1;165(5):1237-51

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