Tg(tetO-UPF1*R844C)3438Kmke
Transgene Detail

Summary

• Symbol: Tg(tetO-UPF1*R844C)3438Kmke
• Name: transgene insertion 3438, Kim M Keeling
• MGI ID: MGI:7787080
• Synonyms: dnUPF1, UPF1-R844C
• Transgene: Tg(tetO-UPF1*R844C)3438Kmke Location: unknown
• Alliance: Tg(tetO-UPF1*R844C)3438Kmke page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(tetO-UPF1*R844C)3438Kmke expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Upf1 (MGI:107995)

• Mutation details: The dnUPF1 transgene consists of a tetracycline response element (TRE) and minimal CMV promoter, hemagglutinin (HA) epitope tag fused to the N terminus human UPF1 cDNA with an R844C mutation followed by SV40 transcriptional terminator (pA). Two funder lines were generated. This is line 3438 with 12 to 14 copies of transgene which shows higher and broader expression. (J:101977, J:295983)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:295983 Echols J, et al., A regulated NMD mouse model supports NMD inhibition as a viable therapeutic option to treat genetic diseases. Dis Model Mech. 2020 Aug 27;13(8):dmm044891
• All: 2 reference(s)