Tg(CD2-Tcra6H9,Cd4-Tcrb6H9)2Dvs
Transgene Detail

Summary

• Symbol: Tg(CD2-Tcra6H9,Cd4-Tcrb6H9)2Dvs
• Name: transgene insertion 2, David Serreze
• MGI ID: MGI:7614246
• Synonyms: DQ8-6H9
• Transgene: Tg(CD2-Tcra6H9,Cd4-Tcrb6H9)2Dvs Location: unknown
• Alliance: Tg(CD2-Tcra6H9,Cd4-Tcrb6H9)2Dvs page

Transgene origin

• Strain of Origin: NOD/ShiLtDvs

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Human TCR and TCR variable Ag recognition sequences isolated from a T1D donor (TCR 6H9, recognizing insulin B9-23 in a DQ8 (HLA-DQA*0301, DQB*0302) or DQ8 trans-restricted) fashion were fused to murine constant regions to create chimeric TCR genes. TCRalpha sequences were inserted into vector pCD2, which drives expression from a human CD2 promoter. TCRbeta sequences were inserted into p428, which drives expression from a murine CD4 promoter. Transgenic fragments were isolated and injected into NOD-cMHCI/II-/- (NOD/ShiLtDvs-H2-K1^d-em1Dvs H2-Ab1^g7-em1Dvs H2-D1^b-em5Dvs/DvsJ) zygotes. The integration site and the copy number of the HLA and TCR transgenes are unknown. This is founder line 2. (J:346039)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:346039 Racine JJ, et al., HLA-DQ8 Supports Development of Insulitis Mediated by Insulin-Reactive Human TCR-Transgenic T Cells in Nonobese Diabetic Mice. J Immunol. 2023 Dec 15;211(12):1792-1805
• All: 1 reference(s)