Tg(Prnp-APPSwInd,KRT14-EGFP)3Dbo
Transgene Detail

Summary

• Symbol: Tg(Prnp-APPSwInd,KRT14-EGFP)3Dbo
• Name: transgenic insertion 3, David Borchelt
• MGI ID: MGI:7541068
• Synonyms: MHSI-695-GFP
• Transgene: Tg(Prnp-APPSwInd,KRT14-EGFP)3Dbo Location: unknown
• Alliance: Tg(Prnp-APPSwInd,KRT14-EGFP)3Dbo page

Transgene origin

• Strain of Origin: (C3H/HeJ x C57BL/6J)F1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(Prnp-APPSwInd,KRT14-EGFP)3Dbo expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APP (351)

  App	(MGI:88059)

  Swedish (KM570/571NL) and Indiana (V617F) mutations

• Mutation details: The mouse amyloid beta (A4) precursor protein (App) sequence was first modified to contain a humanized amyloid-beta (Abeta) domain. This mouse/human chimeric APP (called mo/huAPP695 or APP695) was further mutated to incorporate the Swedish (KM570/571NL) and Indiana (V617F) mutations associated with Alzheimer's disease. This APP695Swe/Ind sequence was placed downstream of the brain-specific mouse prion promoter (Prnp). The transgene was co-integrated with a transgene in which the human KRT14 (K14) promoter drives skin-specific expression of EGFP. (J:340366)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:340366 Xu G, et al., Murine Abeta over-production produces diffuse and compact Alzheimer-type amyloid deposits. Acta Neuropathol Commun. 2015 Nov 14;3:72
• All: 2 reference(s)