Tg(CAG-EGFP,Acr-EGFP)135Osb
Transgene Detail

Summary

• Symbol: Tg(CAG-EGFP,Acr-EGFP)135Osb
• Name: transgene insertion 135, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:7529893
• Synonyms: Tg(CAG/Acr-EGFP)C3-FM135Osb
• Transgene: Tg(CAG-EGFP,Acr-EGFP)135Osb Location: unknown Genetic Position: Chr3, Syntenic
• Alliance: Tg(CAG-EGFP,Acr-EGFP)135Osb page

Transgene origin

• Strain of Origin: C57BL/6NSlc

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-EGFP,Acr-EGFP)135Osb expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Acr (MGI:87884)

• Mutation details: Fertilized oocytes were coinjected with two enhanced green fluorescent protein (EGFP) transgene constructs. One construct, comprising the chicken beta-actin promoter and the cytomegalovirus immediate early enhancer followed by the EGFP coding sequence and the rabbit beta-globin polyadenylation signal, directs expression of EGFP to the cytoplasm of most cell types; sperm of mice having only this construct contain too little cytoplasm to fluoresce. The second construct, containing the mouse preproacrosin promoter and sequences encoding the acrosin signal peptide and N-terminal peptide fused to the EGFP coding sequence, directs EGFP expression specifically to the sperm acrosome, which therefore flouresces. (J:110379)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:110379 Nakanishi T, et al., FISH analysis of 142 EGFP transgene integration sites into the mouse genome. Genomics. 2002 Dec;80(6):564-74
• All: 1 reference(s)