Tg(Met,-lacZ)#Buck
Transgene Detail

Summary

• Symbol: Tg(Met,-lacZ)#Buck
• Name: transgene insertion, Margaret E Buckingham
• MGI ID: MGI:7280777
• Synonyms: Tg(Pax3CSS(5)-TK-nlacZ)#Buck
• Transgene: Tg(Met,-lacZ)#Buck Location: unknown
• Alliance: Tg(Met,-lacZ)#Buck page

Transgene origin

• Strain of Origin: Not Applicable

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: ligonucleotides containing the Pax3 consensus binding site were multimerized. The sequence of the oligonucleotides is as follows: CSSPax3-A, 5-CCTCGTCACGCTTCGAATGT-3; and CSSPax3-B, 5-GGGACATTCGAAGCGTGACGA-3. The oligonucleotides were annealed and polymerized. Concatenamers containing five binding sites were gel-purified, and cloned into a TK-nLacZpA vector, which contains the nlacZ reporter gene under the control of the thymidine kinase (TK) promoter, into an SfiI site located 5 to the TK minimal promoter. A 7.4-kb plasmid fragment for injection was isolated using XhoI and NotI. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)