Tg(HTT*131Q)BACXwy
Transgene Detail

Summary

• Symbol: Tg(HTT*131Q)BACXwy
• Name: transgene insertion BAC, X William Yang
• MGI ID: MGI:6864447
• Synonyms: BAC-CAG
• Transgene: Tg(HTT*131Q)BACXwy Location: unknown Genetic Position: ChrUN, Syntenic
• Alliance: Tg(HTT*131Q)BACXwy page

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The 240 kb human bacterial artificial chromosome (BAC) RP11-866L6, containing the entire 170 kb human Huntingtin (HTT) genomic locus and approximately 20 kb of 5' and 50 kb of 3' flanking sequences, was modified by replacing the human mutant mHTT exon 1 sequence (containing about 120 CAG repeats followed by one CAA, 8 CAGs and another CAA-CAG encoding a total of about 131 glutamine repeats). Founder line BAC-CAG contains 2 tandem copies of the transgene. mHTT repeat sizing and sequencing confirmed an uninterrupted CAG repeat size of about 120, with total polyQ repeat sequence of 131. (J:319519)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:319519 Gu X, et al., Uninterrupted CAG repeat drives striatum-selective transcriptionopathy and nuclear pathogenesis in human Huntingtin BAC mice. Neuron. 2022 Jan 27
• All: 2 reference(s)