Tg(CMV-EGFP,RNU6-RNAi:Nanog)1Ttada
Transgene Detail

Summary

• Symbol: Tg(CMV-EGFP,RNU6-RNAi:Nanog)1Ttada
• Name: transgene insertion 1, Takashi Tada
• MGI ID: MGI:6476745
• Synonyms: NRi-Tg, Tg(CMV-EGFP,U6-shNanog)1Ttada
• Transgene: Tg(CMV-EGFP,RNU6-RNAi:Nanog)1Ttada Location: unknown
• Alliance: Tg(CMV-EGFP,RNU6-RNAi:Nanog)1Ttada page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:154977
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Conditional ready, Knockdown, Reporter)
• Mutation: Insertion
• Mutation details: The transgene contains an RNU6 promoter, floxed CMV promoter driving ubiquitous expression of an EGFP reporter gene, and a short hairpin RNA targeting Nanog. Cre-expression is required to achieve expression of the shRNA. Line 1 was generated. (J:154977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:154977 Yamaguchi S, et al., Conditional knockdown of Nanog induces apoptotic cell death in mouse migrating primordial germ cells. Development. 2009 Dec;136(23):4011-20
• All: 3 reference(s)