Tg(Myh6-COP4*L132C,-lacZ)B5-7Mik
Transgene Detail

Summary

• Symbol: Tg(Myh6-COP4*L132C,-lacZ)B5-7Mik
• Name: transgene insertion B5-7, Michael I Kotlikoff
• MGI ID: MGI:6294062
• Synonyms: aMHC-CatCh-IRES-lacZ, B5-Myh6-CatCh2_IRES_lacZ
• Transgene: Tg(Myh6-COP4*L132C,-lacZ)B5-7Mik Location: unknown
• Alliance: Tg(Myh6-COP4*L132C,-lacZ)B5-7Mik page

Transgene origin

• Strain of Origin: FVB/N X B6(Cg)-Tyr^c-2J/J

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene is designed with (from 5' to 3') a ~5.5 kbp mouse Myh6 minimal promoter sequence (including the 3' most exon of betaMHC, the first three exons of alphaMHC [up to the start codon] as well as intervening sequences), a CatCh-IRES-lacZ-hGHpA construct (CatCh, internal ribosome entry site, beta-galactosidase and a human growth hormone polyadenylation signal). The calcium translocating channelrhodopsin CatCh [ChR2(L132C)] cDNA sequence used encodes the first 309 amino acids of green alga Chlamydomonas reinhardtii channelrhodopsin-2 (ChR2 or cop4) that has been modified with an L132C amino acid substitution (CTC-to-TGC) designed to cause enhanced Ca2+ permeability. Founder line B5-7 was identified with high sensor expression in cardiomyocytes. (J:314125)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858
• All: 1 reference(s)