Tg(CAG-EGFP,-Sumo1,-Sumo2,-Sumo3,-mCherry)10Weiy
Transgene Detail

Summary

• Symbol: Tg(CAG-EGFP,-Sumo1,-Sumo2,-Sumo3,-mCherry)10Weiy
• Name: transgene insertion 10, Wei Yang
• MGI ID: MGI:6150883
• Synonyms: CAG-SUMO
• Transgene: Tg(CAG-EGFP,-Sumo)10Weiy Location: unknown
• Alliance: Tg(CAG-EGFP,-Sumo1,-Sumo2,-Sumo3,-mCherry)10Weiy page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(CAG-EGFP,-Sumo1,-Sumo2,-Sumo3,-mCherry)10Weiy expresses 3 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sumo1 (MGI:1197010)	includes a histidine tag
  mouse	Sumo2 (MGI:2158813)	includes a hemagglutin tag
  mouse	Sumo3 (MGI:1336201)	includes a FLAG tag

• Mutation details: The transgene was designed to have (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, EGFP, a transcriptional/translational STOP cassette (containing a PGK-neo), a second loxP site, a mouse cDNA sequence encoding precursor SUMO1 with N-terminal histidine tag (His-SUMO1), a viral 2A oligopeptide sequence (P2A; mediates ribosomal skipping), a mouse cDNA sequence encoding precursor SUMO2 with N-terminal hemagglutinin tag (HA-SUMO2), a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping), a mouse cDNA sequence encoding precursor SUMO3 with N-terminal FLAG tag (FLAG-SUMO3), a viral 2A oligopeptide sequence (F2A; mediates ribosomal skipping), the monomeric DsRed fluorescent protein variant mCherry gene and a bovine growth hormone polyadenylation signal. Eight founder lines were created, founder line 10 exhibits ubiquitous expression of GFP. (J:260338)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:260338 Yang W, et al., Small ubiquitin-like modifier 3-modified proteome regulated by brain ischemia in novel small ubiquitin-like modifier transgenic mice: putative protective proteins/pathways. Stroke. 2014 Apr;45(4):1115-22
• All: 2 reference(s)