Tg(Il4-AmCyan,Il13-DsRed*)1Wep
Transgene Detail

Summary

• Symbol: Tg(Il4-AmCyan,Il13-DsRed*)1Wep
• Name: transgene insertion 1, William E Paul
• MGI ID: MGI:6118069
• Synonyms: 4C13R
• Transgene: Tg(Il4-AmCyan,Il13-DsRed*)1Wep Location: unknown Genetic Position: Chr10, Syntenic
• Alliance: Tg(Il4-AmCyan,Il13-DsRed*)1Wep page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgene was constructed using BAC recombineering with galK (galactose kinase) selection. BAC clone RP97.23H11 carries the TH2 locus-control region, Il4, Il13 and Kif3a. The ATG start codon of the Il4 gene is targeted with galK, galK is then targeted with AmCyan (Anemonia majano cyan fluorescent protein) sequence. Similarly, the ATG start codon of the Il13 gene is targeted with de-stabilized DsRed (DsRed-DR) via galK selection. AmCyan and DsRed expression is correlated with Il4 and Il3 expression, respectively. (J:197353)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:197353 Roediger B, et al., Cutaneous immunosurveillance and regulation of inflammation by group 2 innate lymphoid cells. Nat Immunol. 2013 Apr 21;14(6):564-573
• All: 10 reference(s)