Tg(GAPDH-OTC/CAT)6424Prab
Transgene Detail

Summary

• Symbol: Tg(GAPDH-OTC/CAT)6424Prab
• Name: transgene insertion 6424, Peter S Rabinovitch
• MGI ID: MGI:5906227
• Synonyms: mCAT BAC
• Transgene: Tg(GAPDH-OTC/CAT)6424Prab Location: unknown
• Alliance: Tg(GAPDH-OTC/CAT)6424Prab page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(GAPDH-OTC/CAT)6424Prab expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CAT (847)

  Cat	(MGI:88271)

  fused to first 25 amino acids of the ornithine transcarbamylase (Otc) leader sequence

• Mutation details: A bacterial artificial chromosome (BAC) library was used to obtain a 110 kb BAC containing the entire human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene. A loxP-flanked STOP signal, followed by a human catalase (CAT) gene, was inserted into this BAC to replace exons 3-7 of GAPDH. The initiating methionine of CAT was deleted, and the first 25 amino acids of the ornithine transcarbamylase (Otc) leader sequence were added to the amino terminus to target this protein product to mitochondria. (J:183593)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:183593 Dai DF, et al., Mitochondrial oxidative stress mediates angiotensin II-induced cardiac hypertrophy and Galphaq overexpression-induced heart failure. Circ Res. 2011 Apr 1;108(7):837-46
• All: 6 reference(s)