Tg(Col2a1-d2EGFP)1Waho
Transgene Detail

Summary

• Symbol: Tg(Col2a1-d2EGFP)1Waho
• Name: transgene insertion 1, William A Horton
• MGI ID: MGI:5904769
• Synonyms: Tg(Col2a1-EGFP*)1Waho
• Transgene: Tg(Col2a1-d2EGFP)1Waho Location: unknown
• Alliance: Tg(Col2a1-d2EGFP)1Waho page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: A construct in which the mouse Col2a1 promoter/enhancer drives expression of d2EGFP was injected into C57BL/6 x SJL F2 embryos. d2EGFP, derived from plasmid pd2EGFP-N1, carries a modification of EGFP in which residues 422-461 of mouse ornithine decarboxylase (MODC) are fused to the C-terminus of EGFP. This modification creates a destabilized, red-shifted variant of the wildtype GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells. (J:169056)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:169056 Tryfonidou MA, et al., Novel type II collagen reporter mice: New tool for assessing collagen 2alpha1 expression in vivo and in vitro. Dev Dyn. 2011 Mar;240(3):663-73
• All: 2 reference(s)