Tg(Afp-lacZ)1Scnch
Transgene Detail

Summary

• Symbol: Tg(Afp-lacZ)1Scnch
• Name: transgene insertion 1, Sophie Conchon
• MGI ID: MGI:5881955
• Synonyms: AFP/BetaGal
• Transgene: Tg(Afp-lacZ)1Scnch Location: unknown
• Alliance: Tg(Afp-lacZ)1Scnch page

Transgene origin

• Strain of Origin: C57BL/6 or CBA or DBA/2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: Mice bearing this transgene express beta-galactosidase (from the E. coli lacZ gene) with a nuclear localization signal driven by a 3299-base pair (bp) fragment of the rat alpha-fetoprotein promoter, encompassing nucleotides -3217 to +102 relative to the transcription start site. This expression construct was inserted into a retroviral plasmid-derived vector in opposite orientation to a neomycin resistance gene under control of the SV40 early promoter. Beta-galactosidase is present at high levels in transgenic fetal liver, where it is restricted to hepatocytes, peaks at birth, and decreases to insignificant levels in the adult liver. (J:239809, J:239810)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

"A transgenic construct...was injected into B6D2 donor eggs" (J:82809) or into "fertilized eggs from B6xCBA F1 hybrids" (J:239809).

References

• Original: J:239809 Cany J, et al., A transgenic mouse with beta-Galactosidase as a fetal liver self-antigen for immunotherapy studies. J Hepatol. 2007 Sep;47(3):396-403
• All: 3 reference(s)