Tg(MMTV-TDGF1)G4Dsal
Transgene Detail

Summary

• Symbol: Tg(MMTV-TDGF1)G4Dsal
• Name: transgene insertion G4, David Salomon
• MGI ID: MGI:5818707
• Synonyms: G4 MMTV-CR-1, MMTV-CR-1, MMTV/CR-1, MMTV-CR-1 G4, MMTV-Cripto-1
• Transgene: Tg(MMTV-TDGF1)G4Dsal Location: unknown
• Alliance: Tg(MMTV-TDGF1)G4Dsal page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(MMTV-TDGF1)G4Dsal expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CRIPTO (6997)

  Cripto

  (MGI:98658)

• Mutation details: Mice bearing this transgene express human teratocarcinoma-derived growth factor 1 (TDGF1; also called Cripto-1 or CR-1) under control of the promoter of the mouse mammary tumor virus (MMTV) long terminal repeat (LTR). The transgene comprises the entire MMTV-LTR followed by the coding sequence of human TDGF1 cDNA, flanked by 220 bp of 5'- and 110 bp of 3'- untranslated region (UTR) DNA, and the simian virus 40 (SV40) intron and polyadenylation signal. Of three transgenic mouse lines expressing human TDGF1 in the mammary gland on day 3 of lactation, line G4 exhibited the highest expression level based on densitometric western blot analysis using a mouse anti-human TDGF1 monoclonal antibody. Human TDGF1 mRNA and protein are also present in other tissues known to be permissive for expression of the MMTV promoter. In 6 month-old transgenic male mice, the protein is detected by immunoblot analysis of lysates of brain, lacrimal gland, lung, and testis and very strongly in epididymus and seminal vesicles. Transgenic females exhibit expression comparable to males' in the brain, lung and lacrimal gland; no TDGF1 protein is detected in the ovary. (J:99542)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:238197 Strizzi L, et al., Epithelial mesenchymal transition is a characteristic of hyperplasias and tumors in mammary gland from MMTV-Cripto-1 transgenic mice. J Cell Physiol. 2004 Nov;201(2):266-76
• All: 3 reference(s)