Tg(Ins2-Chrm3*)SJwe
Transgene Detail

Summary

• Symbol: Tg(Ins2-Chrm3*)SJwe
• Name: transgene insertion S, Jurgen Wess
• MGI ID: MGI:5766547
• Synonyms: beta-R-s Tg
• Transgene: Tg(Ins2-Chrm3*)SJwe Location: unknown
• Alliance: Tg(Ins2-Chrm3*)SJwe page

Transgene origin

• Strain of Origin: C57BL/6NTac

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-Chrm3*)SJwe expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Chrm3	rat/turkey fusion

• Tg(Ins2-Chrm3*)SJwe expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Ins2 (24506)

  Ins2	(MGI:96573)

• Mutation details: The transgene was designed to have (from 5' to 3') a 650 bp rat insulin 2 (Ins2; RIP II) promoter fragment, a hemagglutinin epitope tag (HA), the GsD coding sequence (R-s; described below), and untranslated human growth hormone sequences (containing transcriptional termination, polyadenylation and splicing signals). The GsD sequence is a Galpha>s<-coupled rat M3 DREADD (designer receptor exclusively activated by designer drug). To create the rM3Ds sequence, the wildtype rat muscarinic 3 receptor (Chrm3; M3R) sequence was first modified to replace the second and third intracellular loops with the corresponding turkey beta1-adrenergic receptor (ADRB1 or beta1AR) sequences. The second modification introduced, via site-directed mutagenesis, the Y148C and A238G amino acid substitutions that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). (J:154657)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:154657 Guettier JM, et al., A chemical-genetic approach to study G protein regulation of beta cell function in vivo. Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19197-202
• All: 1 reference(s)