Tg(UBC-GFP,-Fkbp11)HPsou
Transgene Detail

Summary

• Symbol: Tg(UBC-GFP,-Fkbp11)HPsou
• Name: transgene insertion H, Pauline Soulas-Sprauel
• MGI ID: MGI:5699866
• Synonyms: Fkbp11^high, FKBPhigh, Tg(UBC-Fkbp11,-GFP)HPsou
• Transgene: Tg(UBC-GFP,-Fkbp11)HPsou Location: unknown
• Alliance: Tg(UBC-GFP,-Fkbp11)HPsou page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(UBC-GFP,-Fkbp11)HPsou expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Fkbp11 (MGI:1913370)

• Mutation details: This transgene comprises a single, stably integrated copy of a pTRIP deltaU3-derived lentiviral vector containing the coding sequences for green flourescent protein (GFP) and mouse FK506 binding protein 11 (FKBP11, also called FKBP19) under transcriptional control of the human ubiquitin C (UBC) gene promoter. A DNA segment between the GFP and Fbkb11 sequences that encodes a "self-cleaving" picornavirus T2A peptide results in stoichiometric coexpression of the two proteins. Quantitative real-time RT-PCR demonstrated overexpression of Fkbp11 mRNA in unsorted bone marrow cells, splenocytes, isolated splenic B cells and unsorted lymph node cells from Fkbp11^high transgenic mice. Immmunoblot analysis verified overexpression of the FKBP11 protein in splenocytes, splenic sorted B cells and lymph node cells, but the protein was barely detectable in bone marrow cells. (J:227176)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:227176 Ruer-Laventie J, et al., Overexpression of Fkbp11, a feature of lupus B cells, leads to B cell tolerance breakdown and initiates plasma cell differentiation. Immun Inflamm Dis. 2015 Sep;3(3):265-79
• All: 1 reference(s)