Tg(ADCYAP1R1)1Sshe
Transgene Detail

Summary

• Symbol: Tg(ADCYAP1R1)1Sshe
• Name: transgene insertion 1, Sanbing Shen
• MGI ID: MGI:5645747
• Synonyms: A149
• Transgene: Tg(ADCYAP1R1)1Sshe Location: unknown
• Alliance: Tg(ADCYAP1R1)1Sshe page

Transgene origin

• Strain of Origin: C57BL/6 or CBA/Ca

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ADCYAP1R1)1Sshe expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ADCYAP1R1 (117)

  Adcyap1r1

  (MGI:108449)

• Mutation details: The transgenic construct comprised the 130-kb RPCI PAC clone 204D22, containing the human adenylate cyclase activating polypeptide 1 receptor 1 (ADCYAP1R1; also called PAC1) gene, modified by insertion of an internal ribosomal entry site (IRES) followed by the lacZ coding sequence (IRES-lacZ-p[A] cassette) into the 3' untranslated region (3' UTR) downstream of the human gene's polyadenylation signal. Semi-quantitative PCR of genomic DNA from heterozygous mice determined that transgenic line 1 (also called A149) carries six copies of the transgene. RT-PCR of RNA from embryonic day 10.5 (E10.5) embryos revealed a high level of transgene expression. (J:111714)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

"PAC1-overexpressing mice were generated in a mixed genetic background of CBA/Ca: C57Bl/6...." (J:179496)

References

• Original: J:111714 Lang B, et al., Expression of the human PAC1 receptor leads to dose-dependent hydrocephalus-related abnormalities in mice. J Clin Invest. 2006 Jul;116(7):1924-34
• All: 2 reference(s)