Tg(Camk2a-P2rx2/YC3.1)21Khakh
Transgene Detail

Summary

• Symbol: Tg(Camk2a-P2rx2/YC3.1)21Khakh
• Name: transgene insertion 21, Baljit Khakh
• MGI ID: MGI:5569123
• Synonyms: SPRAE
• Transgene: Tg(Camk2a-P2rx2/YC3.1)21Khakh Location: unknown
• Alliance: Tg(Camk2a-P2rx2/YC3.1)21Khakh page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(Camk2a-P2rx2/YC3.1)21Khakh expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  P2rx2 (114115)

  P2rx2

  (MGI:2665170)

• Mutation details: The transgene contains the entire Ca2+/calmodulin-dependent protein kinase II alpha locus, ~110 kbp 5' flanking region (including the Arsi locus and a portion of the Tcof1 locus) and ~50 kbp 3' flanking region (including the Slc6a7 and Cdx1 loci) from BAC RP23-221H5. The P2X2-YC3.1 fusion gene was inserted immediately upstream of the CaMKIIalpha translation initiation codon in exon 1 via homologous recombination/BAC recombineering. The P2rx2/YC3.1 fusion gene encodes rat P2rx2 fused at its carboxy terminus to the fluorescence resonance energy transfer (FRET)-based optical reporter Yellow Cameleon 3.1 (YC3.1). (J:209943)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:209943 Haustein MD, et al., Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway. Neuron. 2014 Apr 16;82(2):413-29
• All: 1 reference(s)