Tg(Cspg4-EGFP*)HDbe
Transgene Detail

Summary

• Symbol: Tg(Cspg4-EGFP*)HDbe
• Name: transgene insertion H, Dwight E Bergles
• MGI ID: MGI:5568765
• Synonyms: NG2-mEGFP, NG2-mEGFP-H
• Transgene: Tg(Cspg4-EGFP*)HDbe Location: unknown
• Alliance: Tg(Cspg4-EGFP*)HDbe page

Transgene origin

• Strain of Origin: B6SJLF1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: Bacterial artificial chromosome (BAC) library (CHORI) was used to obtain a BAC (#RP23-309G21) containing the entire mouse Cspg4 (chondroitin sulfate proteoglycan 4) gene. The BAC was modified to create a membrane anchored form of EGFP (enhanced green fluorescent protein) under the control of the Cspg4 promoter followed by a polyadenylation sequence. EGFP is targeted to the plasma membrane by fusing the first 26 amino acids (containing two palmitoylation domains and a myristoylation domain) of Lck (lymphocyte-specific protein tyrosine kinase) to the EGFP N terminal. (J:209866)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:209866 Hughes EG, et al., Oligodendrocyte progenitors balance growth with self-repulsion to achieve homeostasis in the adult brain. Nat Neurosci. 2013 Jun;16(6):668-76
• All: 7 reference(s)